Luong T K N, Govaerts I, Robben J, Shestakova P, Parac-Vogt T N
Department of Chemistry, KU Leuven, Celestijnenlaan 200F and 200G, 3001 Heverlee, Belgium.
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bontchev Street, Bl.9, 1113 Sofia, Bulgaria.
Chem Commun (Camb). 2017 Jan 3;53(3):617-620. doi: 10.1039/c6cc08555e.
A highly negatively charged binuclear Zr-substituted Keggin polyoxometalate [{α-PWOZr(μ-OH)(HO)}] (ZrK 2 : 2) has been shown to promote the hydrolytic cleavage of phosphoester bonds in the supercoiled plasmid pUC19 DNA under physiological pH and temperature, giving relaxed and linear forms of pUC19 as hydrolysis products. The interaction between ZrK 2 : 2 and DNA was experimentally proven by circular dichroism (CD) spectroscopy and P diffusion ordered NMR spectroscopy.
一种带高负电荷的双核锆取代的Keggin型多金属氧酸盐[{α-PWOZr(μ-OH)(HO)}]（ZrK 2∶2）已被证明在生理pH值和温度下能促进超螺旋质粒pUC19 DNA中磷酸酯键的水解断裂，产生松弛型和线型的pUC19作为水解产物。通过圆二色性（CD）光谱和磷扩散排序核磁共振光谱实验证明了ZrK 2∶2与DNA之间的相互作用。
