优化光谱和空间条件以改善等离子体纳米颗粒的超分辨率成像
Optimization of Spectral and Spatial Conditions to Improve Super-Resolution Imaging of Plasmonic Nanoparticles.
作者信息
De Silva Indrasekara A Swarnapali, Shuang Bo, Hollenhorst Franziska, Hoener Benjamin S, Hoggard Anneli, Chen Sishan, Villarreal Eduardo, Cai Yi-Yu, Kisley Lydia, Derry Paul J, Chang Wei-Shun, Zubarev Eugene R, Ringe Emilie, Link Stephan, Landes Christy F
机构信息
Department of Chemistry, Rice University , 6100 Main Street, MS-60, Houston, Texas 77005, United States.
Department of Materials Science and Nanoengineering, Rice University , 6100 Main Street, MS-325, Houston, Texas 77005, United States.
出版信息
J Phys Chem Lett. 2017 Jan 5;8(1):299-306. doi: 10.1021/acs.jpclett.6b02569. Epub 2016 Dec 22.
Interactions between fluorophores and plasmonic nanoparticles modify the fluorescence intensity, shape, and position of the observed emission pattern, thus inhibiting efforts to optically super-resolve plasmonic nanoparticles. Herein, we investigate the accuracy of localizing dye fluorescence as a function of the spectral and spatial separations between fluorophores (Alexa 647) and gold nanorods (NRs). The distance at which Alexa 647 interacts with NRs is varied by layer-by-layer polyelectrolyte deposition while the spectral separation is tuned by using NRs with varying localized surface plasmon resonance (LSPR) maxima. For resonantly coupled Alexa 647 and NRs, emission to the far field through the NR plasmon is highly prominent, resulting in underestimation of NR sizes. However, we demonstrate that it is possible to improve the accuracy of the emission localization when both the spectral and spatial separations between Alexa 647 and the LSPR are optimized.
荧光团与等离子体纳米颗粒之间的相互作用会改变观察到的发射模式的荧光强度、形状和位置，从而阻碍了对等离子体纳米颗粒进行光学超分辨的努力。在此，我们研究了将染料荧光定位的准确性与荧光团（Alexa 647）和金纳米棒（NRs）之间的光谱和空间间距的函数关系。通过逐层聚电解质沉积来改变Alexa 647与NRs相互作用的距离，同时通过使用具有不同局部表面等离子体共振（LSPR）最大值的NRs来调节光谱间距。对于共振耦合的Alexa 647和NRs，通过NR等离子体向远场的发射非常显著，导致对NR尺寸的低估。然而，我们证明，当Alexa 647与LSPR之间的光谱和空间间距都得到优化时，有可能提高发射定位的准确性。
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