Department of Chemistry, Temple University , Philadelphia, Pennsylvania 19122, United States.
Chem Rev. 2017 Jun 14;117(11):7538-7582. doi: 10.1021/acs.chemrev.6b00547. Epub 2017 Jan 13.
This review describes the growing partnership between super-resolution imaging and plasmonics, by describing the various ways in which the two topics mutually benefit one another to enhance our understanding of the nanoscale world. First, localization-based super-resolution imaging strategies, where molecules are modulated between emissive and nonemissive states and their emission localized, are applied to plasmonic nanoparticle substrates, revealing the hidden shape of the nanoparticles while also mapping local electromagnetic field enhancements and reactivity patterns on their surface. However, these results must be interpreted carefully due to localization errors induced by the interaction between metallic substrates and single fluorophores. Second, plasmonic nanoparticles are explored as image contrast agents for both superlocalization and super-resolution imaging, offering benefits such as high photostability, large signal-to-noise, and distance-dependent spectral features but presenting challenges for localizing individual nanoparticles within a diffraction-limited spot. Finally, the use of plasmon-tailored excitation fields to achieve subdiffraction-limited spatial resolution is discussed, using localized surface plasmons and surface plasmon polaritons to create confined excitation volumes or image magnification to enhance spatial resolution.
这篇综述描述了超分辨率成像和等离子体学之间日益增长的合作关系，描述了这两个主题相互促进以增进我们对纳米世界的理解的各种方式。首先，将基于定位的超分辨率成像策略应用于等离子体纳米粒子基底，其中分子在发光和非发光状态之间被调制，并且它们的发射被定位，从而揭示了纳米粒子的隐藏形状，同时还在其表面上绘制了局部电磁场增强和反应性图案。然而，由于金属基底和单个荧光团之间的相互作用引起的定位误差，必须仔细解释这些结果。其次，等离子体纳米粒子被探索用作超局部化和超分辨率成像的图像对比剂，提供了高光稳定性、大信噪比和距离相关光谱特征等优点，但在定位衍射极限光斑内的单个纳米粒子方面存在挑战。最后，讨论了使用等离子体定制的激发场来实现亚衍射极限空间分辨率的方法，使用局域表面等离子体和表面等离子体极化激元来创建受限的激发体积或图像放大以提高空间分辨率。
