Tamat S R, Moore D E, Allen B J
Department of Pharmacy, University of Sydney, Australia.
Pigment Cell Res. 1989 Jul-Aug;2(4):281-5. doi: 10.1111/j.1600-0749.1989.tb00205.x.
The application of inductively coupled plasma atomic emission spectrometry (ICP-AES) to the determination of the concentration of complex boron-containing compounds in biological tissue samples is described. Tissue digestion is achieved with perchloric acid and hydrogen peroxide in 1 hr at 75 degrees C. The ICP-AES method gave a linear response for elemental boron concentration in the range 0.05 to 100 ppm and does not require the reduction of the boron to a simple species, such as boric acid. Complete recovery of boron in complex boron cluster compounds was obtained. The procedure has been applied to the determination of the boron content in compounds synthesised for neutron capture therapy and is suitable for use in biodistribution studies of such compounds.
描述了电感耦合等离子体原子发射光谱法（ICP-AES）在测定生物组织样品中复杂含硼化合物浓度方面的应用。在75℃下，用高氯酸和过氧化氢在1小时内实现组织消化。ICP-AES方法对元素硼浓度在0.05至100 ppm范围内给出线性响应，并且不需要将硼还原为简单物种，如硼酸。复杂硼簇化合物中的硼实现了完全回收。该程序已应用于中子俘获疗法合成化合物中硼含量的测定，适用于此类化合物的生物分布研究。
