Imperial S, Busquets M, Cortés A, Bozal J
Departament de Bioquimica i Fisiologia, Facultat de Quimica, Universitat de Barcelona, Spain.
Neurochem Res. 1989 Jun;14(6):517-21. doi: 10.1007/BF00964912.
Aspartate aminotransferase from the cytosolic fraction of chicken brain was isolated with acceptable yield and high degree of purity. The enzyme appeared in multiple molecular forms: alpha, beta, gamma, and delta (alpha predominates), as detected by polyacrylamide gel electrophoresis with specific staining. These different forms of the enzyme were separated by DEAE-Sephacel chromatography, and showed different isoelectric points and maximal velocities values, whereas their molecular weight, optimum pH and Michaelis constants were very similar. Generation process studies suggest that minors subforms of the enzyme could be raised from alpha form by a mechanism in which the oxidation of particular amino acid groups are involved.
从鸡脑胞质部分分离出的天冬氨酸转氨酶，其产量可观且纯度很高。通过聚丙烯酰胺凝胶电泳和特异性染色检测发现，该酶呈现出多种分子形式：α、β、γ和δ（α占主导）。这些不同形式的酶通过DEAE - 葡聚糖凝胶色谱法分离，它们显示出不同的等电点和最大速度值，而它们的分子量、最适pH值和米氏常数非常相似。生成过程研究表明，该酶的次要亚基形式可能是通过一种涉及特定氨基酸基团氧化的机制从α形式产生的。
