Oliveira C S, Rodrigues A M, Nogueira G B, Nascimento M A, Punaro G R, Higa E M S
Translational Medicine, Universidade Federal de Sao Paulo, UNIFESP-EPM, Brazil.
Nephrology, Universidade Federal de Sao Paulo, UNIFESP-EPM, Brazil.
Life Sci. 2017 Jan 15;169:37-42. doi: 10.1016/j.lfs.2016.10.028. Epub 2016 Oct 29.
Acute kidney injury is a serious public health problem, especially in intensive care units, where patients may require dialysis support, resulting in 50% mortality.
To evaluate the effects of moderate aerobic exercise on the recovery phase of acute kidney injury induced by gentamicin in rats.
Male adult Wistar rats were allocated into 4 groups: W10+R30, G10+R30, W10+EX30 and G10+EX30; W10 received water (gentamicin vehicle) and G10 received gentamicin for 10days; R30 remained resting and EX30 made exercise for 30days after gentamicin suspension. Training was performed on treadmill. Blood, 24h urine and kidneys were collected for renal function and oxidative stress, antioxidant, TGF-β and histological analysis.
Gentamicin treatment caused decreased renal function significant oxidative stress, reduced urinary nitric oxide and increased TGF-β. G10+R30 presented partial recovery of metabolic data, renal function and lipoperoxidation levels, although they were still altered compared to W10+R30. Besides, we observed the presence of lymphomononuclear infiltrate in the kidneys of G10+R30. G10+EX30 vs G10+R30 showed additional improvement of all the mentioned parameters, showing at histology, regeneration of the tubule epithelium.
Our data suggest that moderate exercises could help in the recovery of metabolic parameters, renal function and structure on gentamicin-induced AKI, perhaps due to restoration of redox balance. This could protect the kidneys from further insults like challenges with nephrotoxic drugs or the aging per se.
急性肾损伤是一个严重的公共卫生问题，尤其是在重症监护病房，患者可能需要透析支持，死亡率达50%。
评估中等强度有氧运动对庆大霉素诱导的大鼠急性肾损伤恢复期的影响。
将成年雄性Wistar大鼠分为4组：W10+R30、G10+R30、W10+EX30和G10+EX30；W10组给予水（庆大霉素溶剂），G10组给予庆大霉素，持续10天；R30组在庆大霉素停用后保持休息状态，EX30组进行30天的运动。训练在跑步机上进行。采集血液、24小时尿液和肾脏样本，进行肾功能、氧化应激、抗氧化、转化生长因子-β（TGF-β）及组织学分析。
庆大霉素治疗导致肾功能下降、显著的氧化应激、尿一氧化氮减少以及TGF-β增加。G10+R30组的代谢数据、肾功能和脂质过氧化水平部分恢复，尽管与W10+R30组相比仍有改变。此外，我们在G10+R30组大鼠的肾脏中观察到淋巴细胞单核细胞浸润。G10+EX30组与G10+R30组相比，上述所有参数均有进一步改善，组织学显示肾小管上皮再生。
我们的数据表明，中等强度运动可能有助于庆大霉素诱导的急性肾损伤的代谢参数、肾功能和结构的恢复，这可能是由于氧化还原平衡的恢复。这可以保护肾脏免受进一步的损伤，如肾毒性药物的挑战或衰老本身。
