Ghazizadeh Hamideh, Fazilati Mohammad, Pasdar Alireza, Avan Amir, Tayefi Maryam, Ghasemi Faeze, Mehramiz Mehraneh, Mirhafez Seyed Reza, Ferns Gordon A, Azimi-Nezhad Mohsen, Ghayour-Mobarhan Majid
Molecular Medicine Group, Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Basic Sciences, Isfahan Payame Noor University, Isfahan, Iran.
Department of Biochemistry, Payame Noor University, Tehran, Iran.
Gene. 2017 Jan 20;598:27-31. doi: 10.1016/j.gene.2016.10.034. Epub 2016 Oct 27.
The metabolic syndrome (MetS) is a clustering of metabolic disorders that is associated with an increased risk of developing cardiovascular-disease, diabetes, and related diseases. Against this background, Vascular Endothelial Growth Factor (VEGF) plays an essential role in angiogenesis, vascular permeability, and hematopoiesis and its increased level is reported to be associated with increasing the risk of developing cardiovascular-disease, stroke and diabetes. Therefore the aim of present study was to explore the association of serum VEGF level and its associated genetic-polymorphism, rs10738760 (A>G) at 9p24.2, in 850 subjects with/without MetS.
MetS was defined according to the International-Diabetes-Federation criteria. Genotyping was carried out using Polymerase chain reaction-amplification refractory mutation system. Anthropometric/biochemical parameters, including FBG, Triglyceride, HDL, TC, etc., were determined followed by univariate and multivariate analyses.
MetS patients had significantly higher levels of BMI, waist-circumference, cholesterol, triglyceride, Hs-CRP and SBP/DBP, while the HDL-C levels was lower in patients group, compared to control group (P<0.05). Moreover, our analysis showed that MetS patients with GA or AA genotypes had a significantly (P=0.03) higher serum level of VEGF.
we demonstrate an association between a VEGF genetic variant with MetS, suggesting its role as a risk stratification factor for MetS.
代谢综合征（MetS）是一组代谢紊乱症候群，与心血管疾病、糖尿病及相关疾病的发病风险增加有关。在此背景下，血管内皮生长因子（VEGF）在血管生成、血管通透性和造血过程中发挥着重要作用，据报道其水平升高与心血管疾病、中风和糖尿病的发病风险增加有关。因此，本研究旨在探讨850例有/无MetS受试者血清VEGF水平及其相关基因多态性（9p24.2处的rs10738760，A>G）之间的关联。
根据国际糖尿病联盟标准定义MetS。采用聚合酶链反应-扩增阻滞突变系统进行基因分型。测定人体测量学/生化参数，包括空腹血糖（FBG）、甘油三酯、高密度脂蛋白（HDL）、总胆固醇（TC）等，随后进行单因素和多因素分析。
与对照组相比，MetS患者的体重指数（BMI）、腰围、胆固醇、甘油三酯、超敏C反应蛋白（Hs-CRP）和收缩压/舒张压水平显著更高，而患者组的HDL-C水平较低（P<0.05）。此外，我们的分析表明，基因型为GA或AA的MetS患者血清VEGF水平显著更高（P=0.03）。
我们证明了VEGF基因变异与MetS之间的关联，表明其作为MetS风险分层因素的作用。
