Dugail I, Quignard-Boulange A, Hoover-Plow J
INSERM U.177, Institut Biomédical des Cordeliers, Paris, France.
Proc Soc Exp Biol Med. 1989 Oct;192(1):61-5. doi: 10.3181/00379727-192-42956.
I mouse strain displays adipocyte hypoplasia responsible for smaller fat pad size compared with C57BL mice. We investigated possible alterations in the proliferation and/or differentiation capacity of preadipocytes from the stroma-vascular fraction of adipose tissue in the I mouse strain. Control C57BL and I mice were studied at 8 weeks of age, and both adipose and stromal cells were isolated from epididymal and inguinal adipose tissue localizations. Results showed that the lower epididymal adipose mass in I mice was accompanied by a decrease in stromal cell number compared with C57BL mice. In inguinal fat pads, total cell number in the stroma-vascular fraction was unmodified; lipoprotein lipase activity significantly increased in stromal cells from I mice compared with control mice. In this depot, further characterization of cells from the stroma-vascular fraction by separation of cells according to density showed an increased number of preadipocytes in the I mouse whole stromal cell population. These preadipocytes seemed unable to undergo terminal maturation, thus leading to a decrease in the number of mature adipocytes. These results indicated that resistance to fat accumulation in I mice is characterized by site-dependent impairment of both the proliferative rate and the differentiation capacity of adipocyte precursors.
与C57BL小鼠相比，I品系小鼠表现出脂肪细胞发育不全，导致脂肪垫尺寸较小。我们研究了I品系小鼠脂肪组织基质血管部分前脂肪细胞增殖和/或分化能力的可能变化。对8周龄的对照C57BL小鼠和I品系小鼠进行研究，从附睾和腹股沟脂肪组织部位分离脂肪细胞和基质细胞。结果显示，与C57BL小鼠相比，I品系小鼠附睾脂肪量较低，同时基质细胞数量减少。在腹股沟脂肪垫中，基质血管部分的总细胞数未改变；与对照小鼠相比，I品系小鼠基质细胞中的脂蛋白脂肪酶活性显著增加。在该部位，通过根据密度分离细胞对基质血管部分的细胞进行进一步表征，结果显示I品系小鼠整个基质细胞群体中的前脂肪细胞数量增加。这些前脂肪细胞似乎无法进行终末成熟，从而导致成熟脂肪细胞数量减少。这些结果表明，I品系小鼠对脂肪积累的抵抗表现为脂肪细胞前体的增殖率和分化能力均存在部位依赖性损伤。
