佐剂增强型mRNA疫苗

Adjuvant-Enhanced mRNA Vaccines.

作者信息

Bialkowski Lukasz, Van der Jeught Kevin, Renmans Dries, van Weijnen Alexia, Heirman Carlo, Keyaerts Marleen, Breckpot Karine, Thielemans Kris

机构信息

Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Laarbeeklaan 103E, Brussels, Belgium.

In Vivo Cellular and Molecular Imaging Laboratory, Vrije Universiteit Brussel, Laarbeeklaan 103K, Brussels, Belgium.

出版信息

Methods Mol Biol. 2017;1499:179-191. doi: 10.1007/978-1-4939-6481-9_11.

DOI:10.1007/978-1-4939-6481-9_11

PMID:27987150

Abstract

Recent advances in molecular biology have led to dramatic enhancement of the stability of in vitro transcribed (IVT) messenger RNA (mRNA) and improvement in its translational efficacy. Nowadays, mRNA-based vaccines represent a promising approach in the field of anticancer immunotherapy, gaining attention over the earlier-established bacteria-, virus-, or cell-based vaccination approaches. Here, we present the experimental procedures employed in our laboratory to induce anticancer immune responses in different murine tumor models using IVT mRNA encoding for immune activation signals and antigens of interest.

摘要

分子生物学的最新进展显著提高了体外转录（IVT）信使核糖核酸（mRNA）的稳定性，并改善了其翻译效率。如今，基于mRNA的疫苗是抗癌免疫疗法领域一种很有前景的方法，比早期建立的基于细菌、病毒或细胞的疫苗接种方法更受关注。在此，我们展示了我们实验室采用的实验程序，即在不同的小鼠肿瘤模型中，使用编码免疫激活信号和感兴趣抗原的IVT mRNA诱导抗癌免疫反应。