Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou 510055, PR China.
School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, PR China.
Mater Sci Eng C Mater Biol Appl. 2017 Feb 1;71:10-16. doi: 10.1016/j.msec.2016.09.070. Epub 2016 Sep 29.
As a means of capitalizing on the synergistic properties between reduced graphene nanosheets (R-GNs) and silver nanoparticles (AgNPs), an efficient and convenient chemical reduction method was used to prepare silver-nanoparticle-decorated reduced graphene nanocomposites (R-GNs/Ag). The products were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), and Raman spectroscopy, which confirmed the loading of well-dispersed silver nanoparticles on reduced graphene sheets. Their antimicrobial activities against oral pathogens such as Candida albicans, Lactobacillus acidophilus, Streptococcus mutans, and Aggregatibacter actinomycetemcomitans were investigated by MIC determination, the counting of colony-forming units (CFU), agar diffusion tests, and growth curve observation. Compared with pure R-GNs and AgNPs, R-GNs/Ag composites exhibited enhanced antimicrobial properties owing to highly dispersed AgNPs on R-GNs.
为了利用还原氧化石墨烯（R-GNs）和银纳米粒子（AgNPs）之间的协同特性，采用一种高效便捷的化学还原法制备了银纳米粒子修饰的还原氧化石墨烯纳米复合材料（R-GNs/Ag）。通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）、X 射线衍射（XRD）和拉曼光谱对产物进行了表征，证实了负载在还原氧化石墨烯片上的银纳米粒子具有良好的分散性。通过 MIC 测定、菌落形成单位（CFU）计数、琼脂扩散试验和生长曲线观察，研究了它们对口腔病原体如白色念珠菌、嗜酸乳杆菌、变形链球菌和伴放线放线杆菌的抗菌活性。与纯 R-GNs 和 AgNPs 相比，由于 R-GNs 上高度分散的 AgNPs，R-GNs/Ag 复合材料表现出增强的抗菌性能。
