Institute of Chemistry, Komi Science Centre, Urals Branch of the Russian Academy of Sciences, Pervomaiskaya St. 48, Syktyvkar 167982, Russia.
Institute of Chemistry, Komi Science Centre, Urals Branch of the Russian Academy of Sciences, Pervomaiskaya St. 48, Syktyvkar 167982, Russia.
Carbohydr Polym. 2017 Feb 10;157:686-694. doi: 10.1016/j.carbpol.2016.10.035. Epub 2016 Oct 17.
Annual dynamics of accumulation and changes in the monosaccharide composition of pectin-, arabinan- and galactan-containing polysaccharides and binding glycans isolated from greenery (thin branches with needles) of Norway spruce were investigated in this study. The polysaccharides were compared with polysaccharides of Siberian fir according to the yields, composition and content of typical components. It was shown that Norway spruce greenery contains lowly methyl-esterified pectin extracted with ammonium oxalate, which is a part of protopectic complex and is bound with components of cell walls via ionic bonds. In contrast, Siberian fir greenery contains mainly water-extracted highly methyl-esterified pectin, weakly bound to cell wall components. It was concluded that an autumn-winter period is the optimal time for harvesting Norway spruce and Siberian fir greenery for isolation of the pectic polysaccharides. The revealed regularities indicate that there is a certain biorhythm of accumulation of the compounds, probably determined by genetic factors.
本研究调查了来自挪威云杉（带针叶的细枝）的果胶、阿拉伯聚糖和半乳聚糖以及结合糖的多糖中单糖组成的积累和变化的年度动态。根据产率、组成和典型成分的含量，将这些多糖与冷杉多糖进行了比较。结果表明，挪威云杉的幼叶含有用草酸铵提取的低甲酯化果胶，它是原果胶复合物的一部分，并通过离子键与细胞壁成分结合。相比之下，西伯利亚冷杉的幼叶主要含有用水提取的高度甲酯化的果胶，与细胞壁成分结合较弱。研究结论认为，秋季至初冬是收获挪威云杉和西伯利亚冷杉幼叶以分离果胶多糖的最佳时间。所揭示的规律表明，这些化合物的积累存在一定的生物节律，可能由遗传因素决定。
