Institute of Chemistry, Komi Science Centre, Urals Branch of the Russian Academy of Sciences, Pervomaiskaya St. 48, Syktyvkar 167982, Russia.
Institute of Chemistry, Komi Science Centre, Urals Branch of the Russian Academy of Sciences, Pervomaiskaya St. 48, Syktyvkar 167982, Russia.
Carbohydr Polym. 2018 Sep 1;195:207-217. doi: 10.1016/j.carbpol.2018.04.074. Epub 2018 Apr 24.
Water-extractable arabinogalactan proteins (AGP) (the main constituent) and pectic polysaccharides were isolated from tree greenery of Picea abies. The carbohydrate part of AGP macromolecules consisted of AG-II, the side chains of which were represented by 1,6- and 1,3,6-β-d-Galp, T-α-l-Araf, 1,3- and 1,5-α-l-Araf, T-β-D-GlcpA and 1,4-β-D-GlcpA, T-α-l-Rhap, T-α-l-Fucp and 4-O-Me-α-l-Fucp residues. It was established that the unusual 4-O-Me-α-l-Fucp monosaccharide are located on the non-reducing ends of the side chains of carbohydrate part of AGP macromolecules, and are bound to 1,4-β-d-GlcpA residues by 1,4-bonds. The backbone of pectin macromolecules consisted mostly of 1,4-α-d-galactopyranosyluronan and RG-I, which side chains were represented by highly branched 1,5-α-l-arabinan. It was shown that RG-I is characterized mainly by short segments, which are alternated with the regions of the non-acetylated and non-methyl-esterified galacturonan. The study revealed that at least a part of the pectin is strongly associated with AGP. It was indicated that the RG-I segments are separated from the AGP-bound pectin by regions of 1,4-α-d-galactopyranosyluronane.
从云杉树绿色部分中分离出了可提取的阿拉伯半乳聚糖蛋白（AGP）（主要成分）和果胶多糖。AGP 大分子的糖部分由 AG-II 组成，其侧链由 1,6-和 1,3,6-β-d-Galp、T-α-l-Araf、1,3-和 1,5-α-l-Araf、T-β-D-GlcpA 和 1,4-β-D-GlcpA、T-α-l-Rhap、T-α-l-Fucp 和 4-O-Me-α-l-Fucp 残基组成。研究发现，这种不寻常的 4-O-Me-α-l-Fucp 单糖位于 AGP 大分子糖部分侧链的非还原端，通过 1,4 键与 1,4-β-d-GlcpA 残基结合。果胶大分子的骨架主要由 1,4-α-d-半乳糖吡喃糖醛酸和 RG-I 组成，其侧链由高度支化的 1,5-α-l-阿拉伯聚糖组成。结果表明，RG-I 主要以短片段为特征，这些片段与非乙酰化和非甲酯化半乳糖醛酸区域交替出现。研究表明，至少一部分果胶与 AGP 强烈相关。研究表明，RG-I 片段通过 1,4-α-d-半乳糖吡喃糖醛酸区域与与 AGP 结合的果胶分离。
