Institute of Chemistry, Komi Science Centre, Ural Branch of the Russian Academy of Sciences, Pervomaiskaya st. 48, Syktyvkar 167982, Russia.
Institute of Chemistry, Komi Science Centre, Ural Branch of the Russian Academy of Sciences, Pervomaiskaya st. 48, Syktyvkar 167982, Russia.
Carbohydr Polym. 2018 Nov 1;199:320-330. doi: 10.1016/j.carbpol.2018.07.027. Epub 2018 Jul 10.
The polysaccharide PA was isolated from tree greenery of spruce Picea abies by the extraction with HCl solution. Monosaccharide analysis PA revealed high content of uronic acid residues (45%), arabinose (19.5%) and galactose (8.6%). It was shown that this polysaccharide contained polymers of various structures. The major component of which were pectic polysaccharides represented mainly by partly methyl-esterified and partly acetylated 1,4-α-D-galacturonan and by minor parts of partly 2-O- and/or 3-O- acetylated rhamnogalacturonan-I (RG-I). The side carbohydrate chains of the branched region of RG-I were represented predominantly by highly branched 1,5-α-L-arabinan and minor portions of 1,4-β-D-galactan. In addition to the dominant pectins, the isolated polysaccharide also contained arabinogalactan proteins (AGP) and binding glycans of the glucuronoarabinoxylan (GAX), glucomannan (GM) and xyloglucan classes, which indicated a close interaction of these polysaccharides in the cell walls. It can be concluded that, at least a part of the pectin is strongly associated with the AGP and binding glycans of the GAX and GM classes. However, neither GM nor GAX were associated with RG-I.
多糖 PA 是从云杉 Picea abies 的树木绿色部分用 HCl 溶液提取得到的。单糖分析表明 PA 含有大量的糖醛酸残基（45%）、阿拉伯糖（19.5%）和半乳糖（8.6%）。结果表明，这种多糖含有各种结构的聚合物。其主要成分是果胶多糖，主要由部分甲酯化和部分乙酰化的 1,4-α-D-半乳糖醛酸和少量的部分 2-O-和/或 3-O-乙酰化鼠李半乳糖醛酸-I（RG-I）组成。RG-I 的支化区的侧碳水化合物链主要由高度支化的 1,5-α-L-阿拉伯聚糖和少量的 1,4-β-D-半乳糖组成。除了主要的果胶外，分离得到的多糖还含有阿拉伯半乳聚糖蛋白（AGP）和结合糖的半乳木聚糖（GAX）、葡甘露聚糖（GM）和木葡聚糖类，这表明这些多糖在细胞壁中密切相互作用。可以得出结论，至少一部分果胶与 AGP 和结合糖的 GAX 和 GM 类强烈相关。然而，GM 或 GAX 都不与 RG-I 相关。
