Takahashi T, Tamura Y, Sawada T, Suzuki S, Muramatsu M, Fujisawa T, Benno Y, Mitsuoka T
National Veterinary Assay Laboratory, Tokyo, Japan.
Res Vet Sci. 1989 Sep;47(2):275-6.
The enzymatic activities of 39 strains of Erysipelothrix rhusiopathiae and 34 of E tonsillae were determined with the API ZYM system. The profiles of these two species were very similar, differing solely in N-acetyl-beta-glucosaminidase activity. Whereas 90 per cent of strains of E rhusiopathiae exhibited strong activity with N-acetyl-beta-glucosaminidase, positive reactions were observed for this enzyme in only 24 per cent of strains of E tonsillae. These results support previous DNA-DNA hybridisation studies and suggest that E tonsillae is a new species of the genus Erysipelothrix.
采用API ZYM系统测定了39株猪丹毒丝菌和34株扁桃体丹毒丝菌的酶活性。这两个菌种的酶谱非常相似,仅在N-乙酰-β-葡萄糖胺酶活性方面有所不同。90%的猪丹毒丝菌菌株对N-乙酰-β-葡萄糖胺酶表现出强活性,而扁桃体丹毒丝菌菌株中只有24%的菌株对该酶呈阳性反应。这些结果支持了先前的DNA-DNA杂交研究,并表明扁桃体丹毒丝菌是丹毒丝菌属的一个新菌种。