Tamura Y, Takahashi T, Zarkasie K, Nakamura M, Yoshimura H
National Veterinary Assay Laboratory, Tokyo, Japan.
Int J Syst Bacteriol. 1993 Jan;43(1):111-4. doi: 10.1099/00207713-43-1-111.
The protein patterns of whole cells of Erysipelothrix rhusiopathiae and Erysipelothrix tonsillarum were studied by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The protein patterns of the 16 strains of E. rhusiopathiae and E. tonsillarum studied, including the type strains of these two species, resembled each other, except that there were 71-, 41-, 34-, and 26-kDa proteins in the E. rhusiopathiae pattern and 74-, 44-, 36-, and 25-kDa proteins in the E. tonsillarum pattern. This observation indicates that there is some phenotypic heterogeneity in the genus Erysipelothrix. In addition, the protein patterns of E. rhusiopathiae serotype reference strains representing serotypes 1 through 23 and type N were compared. The protein patterns of serotype 1a, 1b, 2, 4, 5, 6, 8, 9, 11, 12, 15, 16, 19, and 21 and type N strains were similar to the pattern of the type strain of E. rhusiopathiae (strain ATCC 19414). Conversely, the protein patterns of serotype 3, 7, 10, 14, and 20 strains were very similar to the pattern of the type strain of E. tonsillarum (strain ATCC 43339). An atypical pattern was observed in serotype 13, 17, 18, 22, and 23 strains. These results suggest that this method may be used as an aid in studying the taxonomy of these bacteria.
采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法，对豕链球菌和扁桃体链球菌全细胞的蛋白质图谱进行了研究。所研究的16株豕链球菌和扁桃体链球菌，包括这两个菌种的模式菌株，其蛋白质图谱彼此相似，但豕链球菌图谱中有71 kDa、41 kDa、34 kDa和26 kDa的蛋白质，而扁桃体链球菌图谱中有74 kDa、44 kDa、36 kDa和25 kDa的蛋白质。这一观察结果表明，在链球菌属中存在一些表型异质性。此外，还比较了代表1至23型和N型的豕链球菌血清型参考菌株的蛋白质图谱。1a型、1b型、2型、4型、5型、6型、8型、9型、11型、12型、15型、16型、19型、21型和N型菌株的蛋白质图谱与豕链球菌模式菌株（ATCC 19414菌株）的图谱相似。相反，3型、7型、10型、14型和20型菌株的蛋白质图谱与扁桃体链球菌模式菌株（ATCC 43339菌株）的图谱非常相似。在13型、17型、18型、22型和23型菌株中观察到非典型图谱。这些结果表明，该方法可用于辅助研究这些细菌的分类学。
