鸡软骨和神经视网膜分化过程中DNA序列的扩增
Amplification of DNA sequences during chicken cartilage and neural retina differentiation.
作者信息
Strom C M, Moscona M, Dorfman A
出版信息
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4451-4. doi: 10.1073/pnas.75.9.4451.
Abstract
[3H]BrdUrd-substituted DNA probes were prepared from organ cultures of differentiating chicken neural retina and cell cultures of stage 24 chicken limb buds. Reassociation reactions using the neural retina probe demonstrated amplification of DNA sequences during differentiation of neural retina. This probe also contained sequences present in greater numbers in heart DNA than in DNA from undifferentiated neural retina. Reassociation reactions of both differentiated cartilage and differentiated neural retina DNA with both the neural retina probe and the cartilage probe demonstrated that at least part of the amplified sequences are tissue specific.
摘要
[3H]溴脱氧尿苷取代的DNA探针是从分化中的鸡神经视网膜器官培养物和第24阶段鸡肢芽细胞培养物中制备的。使用神经视网膜探针的重缔合反应表明，在神经视网膜分化过程中DNA序列发生了扩增。该探针还含有在心脏DNA中比在未分化神经视网膜DNA中数量更多的序列。分化的软骨和分化的神经视网膜DNA与神经视网膜探针和软骨探针的重缔合反应表明，至少部分扩增序列是组织特异性的。
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