Kovács Anita, László Kristóf, Zagoracz Olga, Ollmann Tamás, Péczely László, Gálosi Rita, Lénárd László
Institute of Physiology, Pécs University Medical School, Hungary.
Institute of Physiology, Pécs University Medical School, Hungary; Molecular Neurophysiology Research Group, Pécs University, Szentágothai Research Center, Pécs, Hungary.
Neuropeptides. 2017 Apr;62:81-86. doi: 10.1016/j.npep.2016.12.002. Epub 2016 Dec 13.
The amygdaloid body (AMY) plays an important role in memory, learning and reward-related processes. RFRP-1 immunoreactive fibers and NPFF receptors were identified in the AMY, and previously we verified that RFRP-1 infused into the central nucleus of AMY (CeA) induced place preference. The aim of the present study was to examine the possible effects of RFRP-1 in the CeA on passive avoidance learning. Male Wistar rats were examined in two-compartment passive avoidance paradigm. Animals were shocked with 0.5mA current and subsequently were microinjected bilaterally with 50ng or 100ng RFRP-1 in volume of 0.4μl, or 20ng NPFF receptor antagonist RF9 (ANT) alone, or antagonist 15min before 50ng RFRP-1 treatments into the CeA. Fifty nanogram dose of RFRP-1 significantly increased the step-through latency time, the 100ng RFRP-1 and the ANT alone were ineffective. The effect of 50ng RFRP-1 was eliminated by the ANT pretreatment. Our results suggest that intraamygdaloid RFRP-1 enhances learning processes and memory in aversive situations and this effect can specifically be prevented by ANT pretreatment.
杏仁体（AMY）在记忆、学习及与奖赏相关的过程中发挥着重要作用。在杏仁体中发现了RFRP - 1免疫反应性纤维和NPFF受体，并且我们之前证实注入杏仁体中央核（CeA）的RFRP - 1可诱导位置偏爱。本研究的目的是检测CeA中RFRP - 1对被动回避学习的可能影响。雄性Wistar大鼠在两室被动回避范式中接受检测。动物接受0.5mA电流电击，随后双侧微量注射体积为0.4μl的50ng或100ng RFRP - 1，或单独注射20ng NPFF受体拮抗剂RF9（ANT），或在向CeA注射50ng RFRP - 1前15分钟注射拮抗剂。50ng剂量的RFRP - 1显著增加了穿通潜伏期，100ng RFRP - 1和单独的ANT无效。ANT预处理消除了50ng RFRP - 1的作用。我们的结果表明，杏仁体内的RFRP - 1可增强厌恶情境中的学习过程和记忆，并且这种作用可通过ANT预处理特异性地阻断。
