Caldas Ivo Santana, Diniz Livia de Figueiredo, Guedes Paulo Marcos da Matta, Nascimento Álvaro Fernando da Silva do, Galvão Lúcia Maria da Cunha, Lima Wanderson Geraldo de, Caldas Sérgio, Bahia Maria Terezinha
Laboratório de Doenças Parasitárias, Escola de Medicina, Departamento de Ciências Biológicas & Núcleo de Pesquisas em Ciências Biológicas, Universidade Federal de Ouro Preto, Campus Universitário, Morro do Cruzeiro, Ouro Preto, MG, Brazil; Instituto de Ciências Biomédicas, Departamento de Patologia e Parasitologia Básica, Universidade Federal de Alfenas, Alfenas, MG, 37130-001, Brazil.
Instituto de Ciências Biomédicas, Departamento de Patologia e Parasitologia Básica, Universidade Federal de Alfenas, Alfenas, MG, 37130-001, Brazil.
Acta Trop. 2017 Mar;167:40-49. doi: 10.1016/j.actatropica.2016.12.015. Epub 2016 Dec 16.
This study was designed to verify the relationship between IgG antibodies isotypes and myocarditis in Trypanosoma cruzi infection using mice and dogs infected with different T. cruzi strains. The animals were infected with benznidazole-susceptible Berenice-78 and benznidazole-resistant AAS and VL-10 strains. The IgG subtypes were measured in serum samples from dogs (IgG, IgG1, and IgG2) and mice (IgG, IgG1, IgG2a, and IgG2b). The infection of dogs with VL-10 strain induced the highest levels of heart inflammation while intermediate and lower levels were detected with Berenice-78 and AAS strains, respectively. Similar results were found in mice infected with VL-10, but not in those infected with AAS or Berenice-78 strains. The AAS strain induced higher levels of heart inflammation in mice, while Berenice-78 strain was not able to induce it. Correlation analysis between myocarditis and antibody reactivity index revealed very interesting results, mainly for IgG and IgG1, the latter being the most exciting. High IgG1 showed a significant correlation with myocarditis in both experimental models, being more significant in dogs (r=0.94, p<0.0001) than in mice (r=0.58, p=0.047). Overall, our data suggest that IgG1 could be a good marker to demonstrate myocarditis intensity in Chagas disease.
本研究旨在利用感染不同克氏锥虫菌株的小鼠和犬，验证克氏锥虫感染中IgG抗体亚型与心肌炎之间的关系。动物分别感染了对苯硝唑敏感的Berenice - 78株以及对苯硝唑耐药的AAS株和VL - 10株。检测了犬（IgG、IgG1和IgG2）和小鼠（IgG、IgG1、IgG2a和IgG2b）血清样本中的IgG亚型。犬感染VL - 10株引发的心脏炎症水平最高，而感染Berenice - 78株和AAS株分别检测到中度和低度炎症水平。感染VL - 10株的小鼠也得到类似结果，但感染AAS株或Berenice - 78株的小鼠未出现此结果。AAS株在小鼠中引发的心脏炎症水平较高，而Berenice - 78株则无法引发炎症。心肌炎与抗体反应指数之间的相关性分析得出了非常有趣的结果，主要针对IgG和IgG1，后者最为显著。在两个实验模型中，高IgG1水平均与心肌炎显著相关，在犬中更为显著（r = 0.94，p < 0.0001），在小鼠中（r = 0.58，p = 0.047）相对较弱。总体而言，我们的数据表明IgG1可能是显示恰加斯病中心肌炎强度的良好标志物。
