Loganathan Rekha, Nazeer Mehnaz, Goda Vaishnavi, Devaraju Panneer, Ali Mohammed, Karunakaran Premkumar, Jayaraman Megala
Department of Genetic Engineering, SRM University, Kattankulathur, Chennai 603203, India.
Department of Medical Gastroenterology, Rajiv Gandhi Government General Hospital, Chennai 600003, India.
Hum Immunol. 2017 Feb;78(2):216-220. doi: 10.1016/j.humimm.2016.12.002. Epub 2016 Dec 16.
Toll like receptors (TLRs) are a class of molecular pattern recognition receptors, elicits a strong inflammatory immune response against pathogens. Helicobacter pylori (H. pylori), a gram negative flagellate colonizes the human stomach, is responsible for the development of chronic gastritis and gastric carcinoma. The higher incidence of H. pylori infection and gastric cancer in South Indian Tamils demands a genetic study to unravel the influence of TLR4 and TLR9 polymorphisms associated with chronic H. pylori infection. In this study, 230 healthy individuals and 77 patients diagnosed with H. pylori infection were screened for TLR4 (rs1927914, rs4986790, rs4986791) and TLR9 (rs352140, rs34399053, rs150459369) polymorphisms by PCR-RFLP and ARMS-PCR. We observed that the individuals harboring heterozygous and homozygous polymorphic variants of TLR4 conferred a significant risk to develop chronic H. pylori infection and peptic ulcer disease [rs4986790 AG, p=0.001, OR-2.7, 95%CI: 1.5-5.03; GG, p=0.0006, OR-9.8, 95%CI: 2.4-39.4; rs4986791CT, p=0.0001, OR-7.2, 95%CI: 3.7-7.2; TT, p=0.0001, OR-7.9, 95%CI: 2.6-23.7]. Also, the heterozygous variant of TLR9 rs352140, favoured the persistence of the H. pylori infection [p=0.037, OR-1.87, 95%CI: 1.07-3.29]. Thus our findings suggest that TLR4 rs4986790, rs4986791 and TLR9 rs352140 polymorphisms are potential genetic risk factors influencing the disease susceptibility and clinical manifestation of chronic H. pylori infection in Indian Tamils.
Toll样受体（TLRs）是一类分子模式识别受体，可引发针对病原体的强烈炎症免疫反应。幽门螺杆菌（H. pylori）是一种定植于人类胃部的革兰氏阴性鞭毛虫，与慢性胃炎和胃癌的发生有关。南印度泰米尔人中幽门螺杆菌感染和胃癌的高发病率需要进行一项基因研究，以阐明TLR4和TLR9基因多态性与慢性幽门螺杆菌感染的关系。在本研究中，通过聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）和等位基因特异性扩增PCR（ARMS-PCR）对230名健康个体和77名诊断为幽门螺杆菌感染的患者进行了TLR4（rs1927914、rs4986790、rs4986791）和TLR9（rs352140、rs34399053、rs150459369）基因多态性筛查。我们观察到，携带TLR4杂合和纯合多态性变体的个体患慢性幽门螺杆菌感染和消化性溃疡疾病具有显著风险[rs4986790 AG，p = 0.001，OR = 2.7，95%可信区间（CI）：1.5 - 5.03；GG，p = 0.0006，OR = 9.8，95%CI：2.4 - 39.4；rs4986791 CT，p = 0.0001，OR = 7.2，95%CI：3.7 - 7.2；TT，p = 0.0001，OR = 7.9，95%CI：2.6 - 23.7]。此外，TLR9 rs352140的杂合变体有利于幽门螺杆菌感染的持续存在[p = 0.037，OR = 1.87，95%CI：1.07 - 3.29]。因此，我们的研究结果表明，TLR4 rs4986790、rs4986791和TLR9 rs352140基因多态性是影响印度泰米尔人慢性幽门螺杆菌感染疾病易感性和临床表现的潜在遗传危险因素。
