Tourani Mehdi, Habibzadeh Maryam, Shokri-Shirvani Javad, Teymournejad Omid, Mostafazadeh Amrollah, Khafri Soraya, Nouri Hamid Reza
Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
APMIS. 2018 Jan;126(1):76-84. doi: 10.1111/apm.12779. Epub 2017 Nov 14.
Toll-like receptor-4 (TLR4) polymorphisms may influence host immune response against Helicobacter pylori (H. pylori). This study aimed to investigate whether TLR4 polymorphisms are associated with H. pylori susceptibility and risk of peptic ulcer development or not. The TLR4 + 3725 G/C polymorphism was studied using polymerase chain reaction with confronting two-pair primers (PCR-CTPP). In addition, TLR4 Asp299Gly and Thr399Ile polymorphisms were evaluated by PCR-restriction fragment length polymorphism (RFLP). There was no significant difference in TLR4 + 3725 G/C and Asp299Gly genotype frequencies between non-peptic ulcer (NPUD) and peptic ulcer (PUD) individuals in the context of peptic ulcer development and susceptibility to infection with H. pylori. Nevertheless, a significant association with increased risk for PUD development was observed for polymorphism TLR4 Thr399Ile [odds ratio (OR) = 4.2; 95% confidence interval (CI) = 1.35-13.26; p = 0.01]. Correspondingly, TLR4 Thr399Ile polymorphism was associated with H. pylori susceptibility (OR = 0.27; 95% CI = 0.08-0.88; p = 0.04). In addition, TLR4 Thr399Ile polymorphism increased 4.2-fold, the risk of peptic ulcer development in individuals infected by H. pylori carrying CT + TT genotype. Our results showed that TLR4 Thr399Ile polymorphism along with H. pylori infection may play critical roles in peptic ulcer development in North of Iran.
Toll样受体4（TLR4）基因多态性可能影响宿主针对幽门螺杆菌（H. pylori）的免疫反应。本研究旨在调查TLR4基因多态性是否与幽门螺杆菌易感性以及消化性溃疡发生风险相关。采用两对引物扩增阻滞突变系统聚合酶链反应（PCR-CTPP）研究TLR4 +3725 G/C基因多态性。此外，通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）评估TLR4 Asp299Gly和Thr399Ile基因多态性。在消化性溃疡发生及幽门螺杆菌感染易感性方面，非消化性溃疡（NPUD）个体与消化性溃疡（PUD）个体之间，TLR4 +3725 G/C和Asp299Gly基因型频率无显著差异。然而，观察到TLR4 Thr399Ile基因多态性与消化性溃疡发生风险增加显著相关[比值比（OR）=4.2；95%置信区间（CI）=1.35 - 13.26；p = 0.01]。相应地，TLR4 Thr399Ile基因多态性与幽门螺杆菌易感性相关（OR = 0.27；95% CI = 0.08 - 0.88；p = 0.04）。此外，TLR4 Thr399Ile基因多态性使携带CT + TT基因型的幽门螺杆菌感染者发生消化性溃疡的风险增加4.2倍。我们的结果表明，TLR4 Thr399Ile基因多态性与幽门螺杆菌感染可能在伊朗北部消化性溃疡发生中起关键作用。
