Mukherjee Anupam, Nayak Mukti Kant, Dutta Shanta, Panda Samiran, Satpathi Biswa Ranjan, Chawla-Sarkar Mamta
Division of Molecular Virology, National Institute of Cholera and Enteric Diseases, Kolkata, India.
Division of Bacteriology, National Institute of Cholera and Enteric Diseases, Kolkata, India.
PLoS One. 2016 Dec 20;11(12):e0168464. doi: 10.1371/journal.pone.0168464. eCollection 2016.
In 2015, the swine derived A(H1N1)pdm09 pandemic strain outbreak became widespread throughout the different states of India. The reported cases and deaths in 2015 surpassed the previous years with more than 39000 laboratory confirmed cases and a death toll of more than 2500 people. There are relatively limited complete genetic sequences available for this virus from Asian countries. In this study, we describe the full genome analysis of influenza 2015 A(H1N1)pdm09 viruses isolated from West Bengal between January through December 2015. The phylogenetic analysis of the haemagglutinin sequence revealed clustering with globally circulating strains of genogroup 6B. This was further confirmed by the constructed concatenated tree using all eight complete gene segments of Kolkata A(H1N1)pdm09 isolates with the other strains from different timeline and lineages. A study from Massachusetts Institute of Technology (MIT) in 2015 reported novel mutations T200A and D225N in haemagglutinin gene of a 2014 Indian strain (A/India/6427/2014). However, in all the pandemic strains of 2014-2015 reported from India, so far including A(H1N1)pdm09 strains from Kolkata, D225N mutation was not observed, though the T200A mutation was found to be conserved. Neuraminidase gene of the analyzed strains did not show any oseltamivir resistant mutation H275Y suggesting continuation of Tamiflu® as drug of choice. The amino acid sequences of the all gene segments from 2015 A(H1N1)pdm09 isolates identified several new mutations compared to the 2009 A(H1N1)pdm09 strains, which may have contributed towards enhanced virulence, compared to 2009 A(H1N1)pdm09 strains.
2015年，源自猪的甲型H1N1pdm09大流行毒株在印度不同邦广泛爆发。2015年报告的病例和死亡人数超过了前几年，实验室确诊病例超过39000例，死亡人数超过2500人。来自亚洲国家的该病毒完整基因序列相对有限。在本研究中，我们描述了2015年1月至12月从西孟加拉邦分离的甲型H1N1pdm09流感病毒的全基因组分析。血凝素序列的系统发育分析显示与全球流行的6B基因群毒株聚类。使用加尔各答甲型H1N1pdm09分离株的所有八个完整基因片段与来自不同时间线和谱系的其他毒株构建的串联树进一步证实了这一点。2015年麻省理工学院（MIT）的一项研究报告称，2014年印度毒株（A/India/6427/2014）的血凝素基因出现了新的T200A和D225N突变。然而，在印度报告的2014 - 2015年所有大流行毒株中，到目前为止包括来自加尔各答的甲型H1N1pdm09毒株，虽然发现T200A突变是保守的，但未观察到D225N突变。分析毒株的神经氨酸酶基因未显示任何对奥司他韦耐药的H275Y突变，这表明继续将达菲作为首选药物。与2009年甲型H1N1pdm09毒株相比，2015年甲型H1N1pdm09分离株所有基因片段的氨基酸序列鉴定出了几个新突变，这可能导致了与2009年甲型H1N1pdm09毒株相比毒力增强。
