Hansen J B, Wilsgård L, Olsen J O, Svensson B, Osterud B
Institute of Clinical Medicine, University of Tromsø, Norway.
Thromb Haemost. 1989 Jun 30;61(3):493-6.
Heparinized blood sample taken from 81 healthy persons, 40 men and 41 women of whom 17 were on combined oral contraceptives, were incubated with 2 ng lipopolysaccharides (LPS)/ml blood. Quantitation of thromboxane B2 (TxB2) in the resultant plasma revealed a significantly (p less than 0.001) higher generation of TxB2 in men than in women. No sexual differences were observed in thromboplastin activity in unstimulated and LPS stimulated monocytes. Addition of liposomes together with LPS enhanced the synthesis of thromboplastin 2-10 fold and unveiled a higher synthesis of thromboplastin among men. No differences were observed in TxB2 and thromboplastin synthesis in blood collected from females or females using oral contraceptives. A significant correlation between TxB2 and the thromboplastin activity exists within the male (r = 0.46, p = 0.003) and the oral contraceptive (r = 0.57, p = 0.016) group after exposure to LPS. This study suggests that males exposed to LPS may generate more thromboxane A2 than females and thus be at higher risk of developing thrombosis.
从81名健康人（40名男性和41名女性，其中17名服用复方口服避孕药）采集肝素化血样，与2纳克脂多糖（LPS）/毫升血液一起孵育。对所得血浆中血栓素B2（TxB2）的定量分析显示，男性产生的TxB2明显（p<0.001）高于女性。在未刺激和LPS刺激的单核细胞中，凝血活酶活性未观察到性别差异。脂质体与LPS一起添加可使凝血活酶的合成增加2至10倍，并揭示男性中凝血活酶的合成更高。在从女性或服用口服避孕药的女性采集的血液中，TxB2和凝血活酶合成未观察到差异。在暴露于LPS后的男性组（r = 0.46，p = 0.003）和口服避孕药组（r = 0.57，p = 0.016）中，TxB2与凝血活酶活性之间存在显著相关性。这项研究表明，暴露于LPS的男性可能比女性产生更多的血栓素A2，因此发生血栓形成的风险更高。
