Lin W, Guan X, Cao J, Niu B, Chen Q
Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai, China.
J Appl Microbiol. 2017 Mar;122(3):676-685. doi: 10.1111/jam.13384. Epub 2017 Jan 25.
This study focuses on the bactericidal mechanism of the new combination of disinfectant glutaraldehyde-didecyldimethylammonium bromide (GA-DDAB) against Escherichia coli.
Escherichia coli were exposed to GA-DDAB, and assays for cell morphology, K and Ca leakage, H -ATPase activity and DNA degradation were performed. GA-DDAB damaged the cell wall and disrupted cell-membrane integrity. Leakage of K and Ca increased, resulting in significantly lower intracellular concentrations within 60 min of treatment. In addition, H -ATPase was inactivated and DNA was degraded.
Leakage of intracellular components indicated that GA-DDAB damaged the cell membrane of E. coli. This may have caused the observed disruption in equilibria of metal ions, inactivation of H -ATPase, and DNA damage.
Using a low concentration of GA and DDAB, a new combination disinfectant was developed. GA-DDAB displayed higher antimicrobial activity than treatment with GA or DDAB alone. Therefore, GA-DDAB may be a more cost-effective and efficient antimicrobial agent than others in use today. Furthermore, this study provided a paradigm for developing high-efficiency disinfectant to help address the growing problem of bacterial resistance.
本研究聚焦于新型组合消毒剂戊二醛 - 二癸基二甲基溴化铵（GA - DDAB）对大肠杆菌的杀菌机制。
将大肠杆菌暴露于GA - DDAB中，进行细胞形态、钾离子和钙离子泄漏、H⁺ - ATP酶活性及DNA降解检测。GA - DDAB破坏了细胞壁并扰乱了细胞膜完整性。钾离子和钙离子泄漏增加，导致处理60分钟内细胞内浓度显著降低。此外，H⁺ - ATP酶失活且DNA降解。
细胞内成分泄漏表明GA - DDAB破坏了大肠杆菌的细胞膜。这可能导致了所观察到的金属离子平衡紊乱、H⁺ - ATP酶失活及DNA损伤。
使用低浓度的GA和DDAB开发了一种新型组合消毒剂。GA - DDAB比单独使用GA或DDAB表现出更高的抗菌活性。因此，GA - DDAB可能是一种比目前使用的其他消毒剂更具成本效益和高效的抗菌剂。此外，本研究为开发高效消毒剂提供了范例，有助于解决日益严重的细菌耐药问题。
