Li Haimin, Hao Xiaokun, Wang Huimin, Liu Zhengcai, He Yong, Pu Meng, Zhang Hongtao, Yu Hengchao, Duan Juanli, Qu Shibin
Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
Cell Physiol Biochem. 2016;40(6):1334-1344. doi: 10.1159/000453186. Epub 2016 Dec 19.
BACKGROUND/AIMS: Circular RNAs (circRNAs) are a special novel type of a stable, diverse and conserved noncoding RNA in mammalian cells. Particularly in cancer, circRNAs have been reported to be widely involved in the physiological/pathological process of life. However, it is unclear whether circRNAs are specifically involved in pancreatic ductal adenocarcinoma (PDAC).
We investigated the expression profile of circRNAs in six PDAC cancer samples and paired adjacent normal tissues using microarray. A high-throughput circRNA microarray was used to identify dysregulated circular RNAs in six PDAC patients. Bioinformatic analyses were applied to study these differentially expressed circRNAs. Furthermore, quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to confirm these results.
We revealed and confirmed that a number of circRNAs were dysregulated, which suggests a potential role in pancreatic cancer.
this study demonstrates that clusters of circRNAs are aberrantly expressed in PDAC compared with normal samples and provides new potential targets for the future treatment of PDAC and novel insights into PDAC biology.
背景/目的：环状RNA（circRNAs）是哺乳动物细胞中一类特殊的新型稳定、多样且保守的非编码RNA。尤其在癌症中，据报道circRNAs广泛参与生命的生理/病理过程。然而，尚不清楚circRNAs是否特异性参与胰腺导管腺癌（PDAC）。
我们使用微阵列研究了6个PDAC癌组织样本及配对的相邻正常组织中circRNAs的表达谱。采用高通量circRNA微阵列鉴定6例PDAC患者中失调的环状RNA。应用生物信息学分析研究这些差异表达的circRNAs。此外，进行定量逆转录聚合酶链反应（qRT-PCR）以证实这些结果。
我们揭示并证实了许多circRNAs失调，这表明其在胰腺癌中具有潜在作用。
本研究表明，与正常样本相比，circRNAs簇在PDAC中异常表达，为未来PDAC的治疗提供了新的潜在靶点，并为PDAC生物学提供了新的见解。
