Naganuma H, Sasano N
Department of Pathology, Tohoku University School of Medicine, Sendai.
Tohoku J Exp Med. 1989 Jul;158(3):253-62. doi: 10.1620/tjem.158.253.
In order to survey the enzymic activities of steroidogenesis in functioning adrenocortical tumors, we investigated the activities of steroid 21-hydroxylase and 17 alpha-hydroxylase in microsomal fractions of 12 surgically resected adrenocortical tumors associated with Cushing's syndrome (5 adenomas and one carcinoma), primary aldosteronism (5 adenomas) and adrenogenital syndrome (AGS) (one carcinoma), and one adrenocortical hyperplasia resulting from Cushing's disease. Seven adrenal cortices from the patients with mammary carcinoma, renal cell carcinoma or pheochromocytoma were used for normal control. In normal controls 21-hydroxylase activities with progesterone as a substrate were 1.61 +/- 0.25 nmole/min/mg protein and those with 17 alpha-hydroxyprogesterone were 5.22 +/- 1.06 nmole/min/mg protein. The activity of 21-hydroxylase was higher in four cases of 5 aldosteronomas than in normal controls. Those activities in Cushing's adenomas were in the range of normal controls in this study. 17 alpha-hydroxylase activities were much variable from case to case even though in normal controls (4.50 +/- 2.40 nmole/min/mg protein), and in most cases of adenomas 17 alpha-hydroxylase activities were in the range of normal controls. Activities of both hydroxylase in carcinomas were lower than in normal controls. The present paper showed the abnormal steroidogenic enzyme activities in aldosteronomas and adrenocortical carcinomas.
为了研究功能性肾上腺皮质肿瘤中类固醇生成的酶活性，我们调查了12例手术切除的与库欣综合征相关的肾上腺皮质肿瘤（5例腺瘤和1例癌）、原发性醛固酮增多症（5例腺瘤）和肾上腺性征异常综合征（AGS）（1例癌）以及1例由库欣病引起的肾上腺皮质增生的微粒体部分中类固醇21-羟化酶和17α-羟化酶的活性。来自乳腺癌、肾细胞癌或嗜铬细胞瘤患者的7个肾上腺皮质用作正常对照。在正常对照中，以孕酮为底物的21-羟化酶活性为1.61±0.25纳摩尔/分钟/毫克蛋白，以17α-羟孕酮为底物的活性为5.22±1.06纳摩尔/分钟/毫克蛋白。5例醛固酮瘤中有4例的21-羟化酶活性高于正常对照。本研究中库欣腺瘤的这些活性在正常对照范围内。17α-羟化酶活性即使在正常对照中（4.50±2.40纳摩尔/分钟/毫克蛋白）也因病例而异，并且在大多数腺瘤病例中，17α-羟化酶活性在正常对照范围内。癌中两种羟化酶的活性均低于正常对照。本文显示了醛固酮瘤和肾上腺皮质癌中类固醇生成酶活性异常。
