Department of Orthopaedics and Traumatology, University of Turin, Turin, Italy.
Molecular Biotechnology Center, University of Turin, Turin, Italy
J Biol Regul Homeost Agents. 2016 Oct-Dec;30(4 Suppl 1):33-40.
The possible toxic effects of intra-articular tranexamic acid (TA) are still debated. The aim of this study was to evaluate TA effects on human cartilage fragments and synovial biopsies. Explant culture of minced articular cartilage underwent prolonged TA exposure. Histological analysis, immunofluorescence and colorimetric assay for quantification of s-GAG and DNA were performed at the end term. Synoviocytes were cultured for 48h in presence of TA. Light microscopy and flow cytometry analysis were performed at the end of the exposure to TA and one week after the treatment. TA exposure did not influence i) the chondrocyte outgrowth and migration, ii) the expression of chondrogenic and proliferative markers and iii) the s-GAG/DNA ratio. TA treatment did not affect synoviocytes’ morphology and treated cells were phenotypically similar to control cells. This study demonstrated that TA does not negatively affect chondrocytes and synoviocytes cultured in vitro. Thus, our findings may be clinically relevant in order to validate the intra-articular TA administration during orthopedic procedures.
关节内氨甲环酸（TA）的潜在毒性作用仍存在争议。本研究旨在评估 TA 对人软骨碎片和滑膜活检的影响。切下的关节软骨进行了长时间的 TA 暴露培养。在实验结束时进行组织学分析、免疫荧光和定量 s-GAG 和 DNA 的比色分析。在 TA 存在的情况下培养滑膜细胞 48 小时。在 TA 暴露结束时和治疗后一周进行光镜和流式细胞术分析。TA 暴露对以下方面没有影响：i）软骨细胞的生长和迁移；ii）软骨生成和增殖标志物的表达；iii）s-GAG/DNA 比值。TA 处理对滑膜细胞的形态没有影响，且处理后的细胞与对照细胞表型相似。本研究表明，TA 不会对体外培养的软骨细胞和滑膜细胞产生负面影响。因此，我们的发现可能具有临床相关性，以便在骨科手术期间验证关节内 TA 的给药。
