Singh Anjali, Prasad Minakshi, Mishra Bina, Manjunath Siddappa, Sahu Amit Ranjan, Bhuvana Priya G, Wani Sajad Ahmad, Sahoo Aditya Prasad, Kumar Amit, Balodi Shweta, Deora Anupama, Saxena Shikha, Gandham Ravi Kumar
Computational Biology and Genomics Facility Lab, Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, UP-243122, India; Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar, India.
Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar, India.
Genom Data. 2016 Dec 9;11:62-72. doi: 10.1016/j.gdata.2016.12.001. eCollection 2017 Mar.
Bluetongue is an economically important infectious, arthropod borne viral disease of domestic and wild ruminants, caused by Bluetongue virus (BTV). Sheep are considered the most susceptible hosts, while cattle, buffalo and goats serve as reservoirs. The viral pathogenesis of BTV resulting in presence or absence of clinical disease among different hosts is not clearly understood. In the present study, transcriptome of sheep and goats peripheral blood mononuclear cells infected with BTV-16 was explored. The differentially expressed genes (DEGs) identified were found to be significantly enriched for immune system processes - NFκB signaling, MAPK signaling, Ras signaling, NOD signaling, RIG signaling, TNF signaling, TLR signaling, JAK-STAT signaling and VEGF signaling pathways. Greater numbers of DEGs were found to be involved in immune system processes in goats than in sheep. Interestingly, the DEHC (differentially expressed highly connected) gene network was found to be dense in goats than in sheep. Majority of the DEHC genes in the network were upregulated in goats but down-regulated in sheep. The network of differentially expressed immune genes with the other genes further confirmed these findings. Interferon stimulated genes - IFIT1 (ISG56), IFIT2 (ISG54) and IFIT3 (ISG60) responsible for antiviral state in the host were found to be upregulated in both the species. STAT2 was the TF commonly identified to co-regulate the DEGs, with its network showing genes that are downregulated in sheep but upregulated in goats. The genes dysregulated and the networks perturbed in the present study indicate host variability with a positive shift in immune response to BTV in goats than in sheep.
蓝舌病是一种对经济有重要影响的、由节肢动物传播的家养和野生反刍动物病毒性疾病，由蓝舌病病毒（BTV）引起。绵羊被认为是最易感宿主，而牛、水牛和山羊则是病毒储存宿主。BTV在不同宿主中导致临床疾病出现或不出现的病毒发病机制尚不清楚。在本研究中，探究了感染BTV - 16的绵羊和山羊外周血单个核细胞的转录组。发现所鉴定的差异表达基因（DEGs）在免疫系统过程中显著富集——NFκB信号通路、MAPK信号通路、Ras信号通路、NOD信号通路、RIG信号通路、TNF信号通路、TLR信号通路、JAK - STAT信号通路和VEGF信号通路。发现山羊中参与免疫系统过程的DEGs数量比绵羊更多。有趣的是，发现山羊中的差异表达高度连接（DEHC）基因网络比绵羊中的更密集。网络中的大多数DEHC基因在山羊中上调，但在绵羊中下调。差异表达免疫基因与其他基因的网络进一步证实了这些发现。负责宿主抗病毒状态的干扰素刺激基因——IFIT1（ISG56）、IFIT2（ISG54）和IFIT3（ISG60）在两个物种中均上调。STAT2是共同调节DEGs的常见转录因子，其网络显示出在绵羊中下调但在山羊中上调的基因。本研究中失调的基因和受到干扰的网络表明宿主存在变异性，山羊对BTV的免疫反应比绵羊有正向变化。
