School of Medicine, Kunming University, Kunming, Yunnan, China.
School of Medicine and Pharmacy, Ocean University of China, Qingdao, Shandong, China.
Front Immunol. 2022 Dec 1;13:1053059. doi: 10.3389/fimmu.2022.1053059. eCollection 2022.
bluetongue virus (BTV) infection triggers dramatic and complex changes in the host's transcriptional profile to favor its own survival and reproduction. However, there is no whole-transcriptome study of susceptible animal cells with BTV infection, which impedes the in-depth and systematical understanding of the comprehensive characterization of BTV-host interactome, as well as BTV infection and pathogenic mechanisms.
to systematically understand these changes, we performed whole-transcriptome sequencing in BTV serotype 1 (BTV-1)-infected and mock-infected sheep embryonic testicular cells, and subsequently conducted bioinformatics differential analyses.
there were 1504 differentially expressed mRNAs, 78 differentially expressed microRNAs, 872 differentially expressed long non-coding RNAs, and 59 differentially expressed circular RNAs identified in total. Annotation from the Gene Ontology, enrichment from the Kyoto Encyclopedia of Genes and Genomes, and construction of competing endogenous RNA networks revealed differentially expressed RNAs primarily related to virus-sensing and signaling transduction pathways, antiviral and immune responses, inflammation, and development and metabolism related pathways. Furthermore, a protein-protein interaction network analysis found that BTV may contribute to abnormal spermatogenesis by reducing steroid biosynthesis. Finally, real-time quantitative PCR and western blotting results showed that the expression trends of differentially expressed RNAs were consistent with the whole-transcriptome sequencing data.
this study provides more insights of comprehensive characterization of BTV-host interactome, and BTV infection and pathogenic mechanisms.
蓝舌病毒（BTV）感染会引发宿主转录谱的剧烈和复杂变化，以利于自身的存活和繁殖。然而，目前还没有对易感动物细胞进行 BTV 感染的全转录组研究，这阻碍了对 BTV-宿主互作组的全面特征、BTV 感染和致病机制的深入和系统理解。
为了系统地了解这些变化，我们对 BTV 血清型 1（BTV-1）感染和模拟感染的绵羊胚胎睾丸细胞进行了全转录组测序，随后进行了生物信息学差异分析。
总共鉴定出 1504 个差异表达的 mRNA、78 个差异表达的 microRNA、872 个差异表达的长非编码 RNA 和 59 个差异表达的环状 RNA。基因本体论注释、京都基因与基因组百科全书富集以及竞争性内源 RNA 网络构建表明，差异表达的 RNA 主要与病毒感应和信号转导途径、抗病毒和免疫反应、炎症以及发育和代谢相关途径相关。此外，蛋白质-蛋白质相互作用网络分析发现，BTV 可能通过减少类固醇生物合成导致精子发生异常。最后，实时定量 PCR 和 Western blot 结果表明，差异表达 RNA 的表达趋势与全转录组测序数据一致。
本研究为 BTV-宿主互作组的全面特征、BTV 感染和致病机制提供了更深入的见解。
