Vieira Frez Flávia Cristina, Martins Colombo Perles Juliana Vanessa, Robert Linden David, Gibbons Simon John, Amilcar Martins Heber, Almeida Brito Romualdo Débora, de Souza Sara Raquel, Daion Piovezana Bossolani Gleison, Zanoni Jacqueline Nelisis
Morphological Science, State University of Maringá, Brazil.
Department of Morphological Sciences, State University of Maringá.
Rev Esp Enferm Dig. 2017 Mar;109(3):190-195. doi: 10.17235/reed.2016.4338/2016.
Interstitial cells of Cajal (ICC) are required for normal motility in the gastrointestinal tract. Depletion of ICC has been associated with diabetic gastroenteropathy.
To determine the effect of quercertin supplementation on anoctamin-1 (Ano1) immunoreactive ICC in the myenteric region (ICC-MY) and deep muscular plexus (ICC-DMP) in the jejunum of diabetic rats.
Thirty-two 90-day-old male Wistar rats were distributed into the following groups: normoglycemic (C), normoglycemic supplemented with quercetin (CQ; 40 mg daily), diabetic (D), and diabetic supplemented with quercetin (DQ; 40 mg daily). Diabetes was induced by streptozotocin injection. After 120 days, preparations of the jejunal muscular and submucosal layers were immunostained for Ano1 to visualize ICC. Evaluation of the immunofluorescence intensity as well as density of ICC was performed.
The density of ICC-MY was 46% lower in group D compared to group C (p < 0.01); ICC-DMP were reduced by 37% (p > 0.05). After quercertin treatment, the densities of ICC-MY were significantly higher in the DQ group compared to group D (ICC-MY: 58%, p < 0.05). Supplementation with quercetin in normoglycemic animals (CQ) compared with group C did not significantly change the ICC density (p > 0.05).
In STZ-treated diabetic rats, diabetes promoted a reduction in the density of jejunal ICC-MY with no significant effect on ICC-DMP. Supplementation with quercetin (DQ) appeared to protect ICC-MY from depletion in diabetes possibly due to its antioxidant action.
胃肠道正常蠕动需要Cajal间质细胞（ICC）。ICC的减少与糖尿病性胃肠病有关。
确定补充槲皮素对糖尿病大鼠空肠肌间神经丛（ICC-MY）和深部肌丛（ICC-DMP）中anoctamin-1（Ano1）免疫反应性ICC的影响。
将32只90日龄雄性Wistar大鼠分为以下几组：正常血糖组（C）、补充槲皮素的正常血糖组（CQ；每日40mg）、糖尿病组（D）和补充槲皮素的糖尿病组（DQ；每日40mg）。通过注射链脲佐菌素诱导糖尿病。120天后，对空肠肌层和黏膜下层进行免疫染色以观察Ano1，从而可视化ICC。对免疫荧光强度以及ICC密度进行评估。
与C组相比，D组ICC-MY的密度降低了46%（p<0.01）；ICC-DMP减少了37%（p>0.05）。槲皮素治疗后，DQ组ICC-MY的密度显著高于D组（ICC-MY：58%，p<0.05）。与C组相比，正常血糖动物补充槲皮素（CQ）并未显著改变ICC密度（p>0.05）。
在经链脲佐菌素处理的糖尿病大鼠中，糖尿病导致空肠ICC-MY密度降低，对ICC-DMP无显著影响。补充槲皮素（DQ）似乎可保护ICC-MY在糖尿病中不被消耗，这可能归因于其抗氧化作用。
