[分枝杆菌及相关细菌全细胞霉菌酸甲醇裂解物的薄层色谱分析]

[Thin-layer chromatography of mycolic acids methanolysates of whole-cell in Mycobacterium and related bacteria].

作者信息

Zhuang Y H, Zhang Y, Han Y H, Liu Z H, Ruan J S

出版信息

Wei Sheng Wu Xue Bao. 1989 Feb;29(1):15-9.

Abstract

Mycolic acid methanolysates of whole-cell in Mycobacterium and related bacteria were analysed by thin-layer chromatography. The experimental results show that five of twenty-two species, M. tuberculosis, M. bovis, M. kansasii, M. marinum and M. gastri have similar pattern of mycolates, composed of alpha-mycolates, methoxymycolates, ketomycolates and two unknown components. M. gilvum, M. phleri, M. avium, M. intracellulare, M. xenopi and M. nonchromogenicum contain alpha-mycolates, ketomycolates and wax-ester. The patterns of TLC for other tested species were different from each other. Nocardia, Rhodococcus and Corynebacterium show a relatively simple pattern which principally contain alpha-mycolates. The four genus can be differentiated. Spots of mycolic acids of nine strains Mycobacterium sp. isolated from patients in this hospital were similar to M. tuberculosis. These strains were also identified to the same result as above by traditional methods. The method is of value in the classification and identification of Mycobacterium.

摘要

采用薄层色谱法分析了分枝杆菌及相关细菌全细胞的霉菌酸甲醇解产物。实验结果表明，22个菌种中的5个，即结核分枝杆菌、牛分枝杆菌、堪萨斯分枝杆菌、海分枝杆菌和胃分枝杆菌，具有相似的霉菌酸模式，由α-霉菌酸、甲氧基霉菌酸、酮基霉菌酸和两种未知成分组成。吉尔万分枝杆菌、弗氏分枝杆菌、鸟分枝杆菌、胞内分枝杆菌、蟾蜍分枝杆菌和非产色分枝杆菌含有α-霉菌酸、酮基霉菌酸和蜡酯。其他受试菌种的薄层色谱模式彼此不同。诺卡氏菌、红球菌和棒状杆菌显示出相对简单的模式，主要含有α-霉菌酸。这四个属可以区分。从本院患者中分离出的9株分枝杆菌属菌株的霉菌酸斑点与结核分枝杆菌相似。通过传统方法对这些菌株的鉴定结果也与上述相同。该方法在分枝杆菌的分类和鉴定中具有重要价值。