Goodfellow M, Collins M D, Minnikin D E
J Gen Microbiol. 1976 Oct;96(2):351-8. doi: 10.1099/00221287-96-2-351.
Acid methanolysates of strains representing 58 coryneform taxa were examined for mycolic acids and other long-chain constituents by thin-layer chromatography. Mycolic esters were detected in the methanolysates of true corynebacteria but not in those from plant pathogenic bacteria, Corynebacterium haemolyticum, Corynebacterium pyogenes or from representatives of the genera Arthrobacter, Cellulomonas, Curtobacterium, Kurthia or Oerskovia. Thin-layer chromatography of whole-organism methanolysates provides a simple method for distinguishing true corynebacteria from coryneforms which do not contain mycolic acids, and from nocardiae and mycobacteria which produce mycolic acids of different mobility. At present the mycolic esters of true corynebacteria cannot be clearly separated from those of some rhodochrous strains.
通过薄层色谱法对代表58个棒状杆菌类群的菌株的酸性甲醇裂解物进行了分枝菌酸和其他长链成分的检测。在真正的棒状杆菌的甲醇裂解物中检测到了分枝菌酸酯，但在植物病原菌、溶血棒状杆菌、化脓棒状杆菌或节杆菌属、纤维单胞菌属、短小杆菌属、库特氏菌属或厄氏菌属代表菌株的甲醇裂解物中未检测到。全菌体甲醇裂解物的薄层色谱法提供了一种简单的方法，可将真正的棒状杆菌与不含分枝菌酸的棒状杆菌、以及与产生迁移率不同的分枝菌酸的诺卡氏菌和分枝杆菌区分开来。目前，真正的棒状杆菌的分枝菌酸酯无法与一些红球菌菌株的分枝菌酸酯清楚地分离。
