麻风分枝杆菌及相关菌种的霉菌酸甲酯薄层色谱图谱的比较研究。

Comparative study on the thin-layer chromatographic pattern of methyl mycolates of M. leprae and related species.

作者信息

Datta A K, Katoch V M, Sharma V D, Katoch K

出版信息

Int J Lepr Other Mycobact Dis. 1985 Mar;53(1):45-51.

Abstract

The thin-layer chromatographic (TLC) pattern of methyl mycolates of Mycobacterium leprae has been compared with the lipids extracted from M. leprae-infected tissue material and also with those of other cultivable mycobacterial species. It has been found that M. leprae-derived methyl mycolates give two spots, namely, alpha- and keto-derivatives, after charring the TLC plates with K2Cr2O7/H2SO4 spray. Lipids extracted from infected tissue material also show spots with the same Rf values when compared with those of armadillo-derived M. leprae. Normal skin tissue extracts, however, do not show any presence of mycolates. This TLC pattern of M. leprae-derived methyl mycolates is characteristic and is not shown by any other mycobacterial species isolated from leprosy lesions. The strain and species which have been earlier found to resemble M. leprae antigenically or biochemically could readily be distinguished with the help of the TLC pattern of their methyl mycolates.

摘要

已将麻风分枝杆菌的霉菌酸甲酯的薄层色谱（TLC）图谱与从麻风分枝杆菌感染的组织材料中提取的脂质以及其他可培养分枝杆菌物种的脂质进行了比较。结果发现，用K2Cr2O7/H2SO4喷雾使TLC板炭化后，麻风分枝杆菌来源的霉菌酸甲酯产生两个斑点，即α-和酮衍生物。与犰狳来源的麻风分枝杆菌相比，从感染组织材料中提取的脂质也显示出具有相同Rf值的斑点。然而，正常皮肤组织提取物未显示任何霉菌酸的存在。麻风分枝杆菌来源的霉菌酸甲酯的这种TLC图谱具有特征性，从麻风病变中分离出的任何其他分枝杆菌物种都不具有这种图谱。早期发现的在抗原性或生化特性上与麻风分枝杆菌相似的菌株和物种，可以借助其霉菌酸甲酯的TLC图谱很容易地加以区分。