Wilmańska D, Milczarek K, Rumijowska A, Bartnicka K, Sedlaczek L
Institute of Microbiology, University of Lódź, Poland.
Appl Microbiol Biotechnol. 1992 Aug;37(5):626-30. doi: 10.1007/BF00240738.
Stable mutants showing improved 11-hydroxylation of Substance S were isolated, following treatment with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) and regeneration of uninucleate protoplasts of the appropriate fungal strains. This procedure was especially suitable for obtaining more directed 11 beta-hydroxylation of Substance S with Curvularia lunata IM 2901. Apart from producing cortisol (11 beta-hydroxy-S), the parent strain formed several by-products that significantly lowered the yield of the desired 11 beta-hydroxyderivative. Isolated mutants of this microorganism carried out directed 11 beta-hydroxylation with only a small amount of one of the by-products, which resulted in a much higher yield of cortisol.
在用N-甲基-N'-硝基-N-亚硝基胍（NTG）处理并使合适的真菌菌株单核原生质体再生后，分离出了显示出物质S的11-羟化作用得到改善的稳定突变体。该方法特别适用于新月弯孢霉IM 2901对物质S进行更有针对性的11β-羟化。除了产生皮质醇（11β-羟基-S）外，亲本菌株还形成了几种副产物，这些副产物显著降低了所需11β-羟基衍生物的产量。该微生物的分离突变体仅产生少量副产物之一进行有针对性的11β-羟化，从而使皮质醇的产量大大提高。
