Sánchez-Soto Eduardo, Ponce-Ramos Rosa, Hernández-Gutiérrez Rodolfo, Gutiérrez-Ortega Abel, Álvarez Angel H, Martínez-Velázquez Moisés, Absalón Angel E, Ortiz-Lazareno Pablo, Limón-Flores Alberto, Estrada-Chávez Ciro, Herrera-Rodríguez Sara E
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Jalisco, (CIATEJ-CONACYT), Unidad de Biotecnología Médica y Farmacéutica, Avenida Normalistas 800, Colinas de la normal, Guadalajara, Jalisco, C.P. 44270, Mexico.
Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Jalisco, (CIATEJ-CONACYT), Unidad de Biotecnología Médica y Farmacéutica, Avenida Normalistas 800, Colinas de la normal, Guadalajara, Jalisco, C.P. 44270, Mexico.
Microb Pathog. 2017 Feb;103:57-64. doi: 10.1016/j.micpath.2016.12.007. Epub 2016 Dec 19.
Bovine colostrum contains compounds, which provide passive immune protection from mother to newborn calves. Little is known about cytokine levels and their role in bovine colostrum. Moreover, the capacity of bovine colostrum cells to mount specific immune responses after natural exposure to bovine tuberculosis (bTB) antigens in dairy herds has not been studied, thus far. The purpose of this study was to identify biomarkers for bTB infection measurable in bovine colostrum. The present study reveals that isolated-immune colostrum cells can mount a specific immune response against bTB antigens, by measuring the novo IFN-γ release in cell culture. We found that IFN-γ levels in the responders (Bov) to bTB antigen were higher than in non-responders (Bov). On the other hand, proinflammatory cytokines contained in colostrum's whey were tested in Tuberculin Skin Test (TST) reactor (TST) and non-reactor (TST) animals to assess their potential role as biomarker. We observed that IFN-γ levels were lower or undetectable, as opposed to IL4 levels were measurable, the TNF-α level was higher in TST than TST, while IL-6 levels showed the opposite reaction and with no statistical significance. Moreover, IL-1α mRNA expression levels were higher in colostrum mononuclear cells (CMC) in Bov cattle. Collectively, these data suggest that the differential expression of pro and anti-inflammatory cytokines could have relevant value to diagnose bTB in cattle.
牛初乳含有多种化合物，可为新生小牛提供被动免疫保护。目前对牛初乳中的细胞因子水平及其作用了解甚少。此外，迄今为止，尚未研究奶牛群自然接触牛结核病（bTB）抗原后牛初乳细胞产生特异性免疫反应的能力。本研究的目的是确定可在牛初乳中检测到的牛结核病感染生物标志物。本研究表明，通过测量细胞培养中新生IFN-γ的释放，分离的免疫初乳细胞可对bTB抗原产生特异性免疫反应。我们发现，对bTB抗原产生反应的（Bov）细胞中的IFN-γ水平高于无反应的（Bov）细胞。另一方面，对结核菌素皮肤试验（TST）反应者（TST）和无反应者（TST）动物的初乳乳清中所含的促炎细胞因子进行了检测，以评估它们作为生物标志物的潜在作用。我们观察到，与可测量的IL4水平相反，IFN-γ水平较低或无法检测到，TST中的TNF-α水平高于TST，而IL-6水平则呈现相反反应且无统计学意义。此外，Bov牛初乳单核细胞（CMC）中的IL-1α mRNA表达水平较高。总体而言，这些数据表明，促炎和抗炎细胞因子的差异表达可能对牛结核病的诊断具有重要价值。
