Mo Ting, Yue Shuangbing, Tian Huan, Lin Hong, Zhang Guanglu, Zhang Zili
Department of Integrated Chinese and Western Medicine, Shenzhen Second People's Hospital (Shenzhen University First Affiliated Hospital), Shenzhen, Guangdong, China (mainland).
Med Sci Monit. 2016 Dec 23;22:5068-5073. doi: 10.12659/msm.898685.
BACKGROUND Previous research showed that granulized Fu-Zheng-Xiao-Liu has a significant effect on breast cancer. However, it remains unclear whether HER-2 plays a role in this anti-cancer effect. MATERIAL AND METHODS Serum of male SD rats administered Fu-Zheng-Xiao-Liu granules (SF) was prepared and used to treat HER-2 positive breast cancer cell line SKBR-3. PBS and herceptin were used as negative and positive controls, respectively. MTT was used to detect the proliferation of SKBR-3 cells. Flow cytometry was used to measure the apoptosis of SKBR-3 cells. Western blot and immunofluorescence were used to measure the expression change of HER-2. RESULTS Serum of male SD rats administered Fu-Zheng-Xiao-Liu granules had significantly reduced HER-2 expression at both mRNA level and protein level, significantly inhibited proliferation of SKBR-3 cells, and significantly increased apoptosis of SKBR-3 cells, compared to that of the blank control group or serum control group. CONCLUSIONS Fu-Zheng-Xiao-Liu granules affect proliferation and apoptosis through inhibition of HER-2 transcription and translation, providing an experimental basis for further study of the mechanism by which Fu-Zheng-Xiao-Liu granules affect breast cancer.
背景 先前的研究表明，扶正消瘤颗粒对乳腺癌有显著疗效。然而，HER-2是否在这种抗癌作用中发挥作用仍不清楚。材料与方法 制备给予扶正消瘤颗粒（SF）的雄性SD大鼠血清，并用于处理HER-2阳性乳腺癌细胞系SKBR-3。PBS和赫赛汀分别用作阴性和阳性对照。采用MTT法检测SKBR-3细胞的增殖情况。采用流式细胞术检测SKBR-3细胞的凋亡情况。采用蛋白质免疫印迹法和免疫荧光法检测HER-2的表达变化。结果 与空白对照组或血清对照组相比，给予扶正消瘤颗粒的雄性SD大鼠血清在mRNA水平和蛋白质水平均显著降低了HER-2的表达，显著抑制了SKBR-3细胞的增殖，并显著增加了SKBR-3细胞的凋亡。结论 扶正消瘤颗粒通过抑制HER-2的转录和翻译影响细胞增殖和凋亡，为进一步研究扶正消瘤颗粒影响乳腺癌的机制提供了实验依据。
