Pollott Geoff, Brito Amanda, Gardiner Christopher, Lawson Charlotte
Department of Production and Population Health, Royal Veterinary College, London, UK.
Department of Comparative Biomedical Sciences, Royal Veterinary College, London, UK.
Biomark Insights. 2016 Dec 13;11:147-155. doi: 10.4137/BMI.S38438. eCollection 2016.
Cow's milk is economically important to the agricultural industry with the nutritive value of milk being routinely measured. This does not give full insight into normal mammary tissue turnover during the course of lactation, which could be important for both an understanding of milk production and animal welfare. We have previously demonstrated that submicron particles, including extracellular vesicles (EVs), can be measured in unprocessed cow's milk by flow cytometry and that they correlate with stage of lactation. A number of different techniques are available to measure EVs and other milk-derived particles. The purpose of this study was to compare two different methodologies and the value of fluorescent staining for the phospholipid phosphatidylserine (PS), which is exposed on the surface of EVs (but not other milk-derived particles). We used two different flow cytometers and nanotracker analysis to detect milk-derived particles in whole and skimmed milk samples. Our findings indicate significant correlation, after staining for PS, suggesting potential for larger multicenter studies in the future.
牛奶对农业产业具有重要的经济意义，其营养价值会定期进行测定。但这并不能全面洞察泌乳过程中正常乳腺组织的更新情况，而这对于理解牛奶生产和动物福利都可能很重要。我们之前已经证明，通过流式细胞术可以在未加工的牛奶中检测到亚微米颗粒，包括细胞外囊泡（EVs），并且它们与泌乳阶段相关。有多种不同技术可用于测量EVs和其他源自牛奶的颗粒。本研究的目的是比较两种不同的方法以及对暴露于EVs表面（而非其他源自牛奶的颗粒）的磷脂磷脂酰丝氨酸（PS）进行荧光染色的价值。我们使用了两种不同的流式细胞仪和纳米追踪分析来检测全脂和脱脂牛奶样品中源自牛奶的颗粒。我们的研究结果表明，在对PS进行染色后存在显著相关性，这表明未来有开展更大规模多中心研究的潜力。
