Karim Md Ziaul, Uesugi Daisuke, Nakayama Noriyuki, Hossain M Monzur, Ishihara Kohji, Hamada Hiroki
Laboratory of Food Preventive Medicine, Department of Life Science, Faculty of Science, Okayama University of Science, Okayama, Japan.
Professor and Director, Institute of Biological Sciences, University of Rajshahi, Rajshahi, Bangladesh.
Biochem Insights. 2016 Dec 18;8(Suppl 2):33-37. doi: 10.4137/BCI.S30378. eCollection 2015.
Stevioside is a natural sweetener from Stevia leaf, which is 300 times sweeter than sugar. It helps to reduce blood sugar levels dramatically and thus can be of benefit to diabetic people. Tissue culture is a very potential modern technology that can be used in large-scale disease-free stevia production throughout the year. We successfully produced stevia plant through in vitro culture for identification of stevioside in this experiment. The present study describes a potential method for identification of stevioside from tissue culture-derived stevia leaf. Stevioside in the sample was identified using HPLC by measuring the retention time. The percentage of stevioside content in the leaf samples was found to be 9.6%. This identification method can be used for commercial production and industrialization of stevia through in vitro culture across the world.
甜菊糖苷是一种从甜叶菊叶子中提取的天然甜味剂，其甜度是糖的300倍。它有助于显著降低血糖水平，因此对糖尿病患者有益。组织培养是一项极具潜力的现代技术，可用于全年大规模生产无病害的甜叶菊。在本实验中，我们通过体外培养成功培育出甜叶菊植株以鉴定甜菊糖苷。本研究描述了一种从组织培养所得甜叶菊叶片中鉴定甜菊糖苷的潜在方法。通过测量保留时间，使用高效液相色谱法（HPLC）对样品中的甜菊糖苷进行鉴定。发现叶片样品中甜菊糖苷的含量百分比为9.6%。这种鉴定方法可用于全球范围内通过体外培养实现甜叶菊的商业化生产和产业化。
