Stanovnik Lovro
Faculty of Medicine, Dept. of Pharmacology, Korytkova 2, 1000 Ljubljana, Slovenia, Slovenia.
Pflugers Arch. 2000 Jan;440(Suppl 1):R197-R199. doi: 10.1007/s004240000063.
In our previous experiments measuring acid secretion in the isolated mouse stomach we found higher EC values (agonist concentration, evoking half maximal effect) for histamine than in the experiments on the isolated guinea pig right atrium (contraction frequency). The difference could be due to nonequilibrium conditions in the isolated mouse stomach preparation or to species differences. In order to check this possibility and to test the consistency of concentration-response relationship (CRR) parameter determination on an isolated secretory organ, we compared EC for histamine and pA for famotidine on the two isolated preparations from the same species, guinea pigs.Both, EC and pA values were significantly different when measuring gastric acid secretion (9.7 μM and 6.07) from the parameters, found in the isolated right atrium measuring frequency of contractions (1.6 μM and 7.06).From these results it can be concluded that the isolated portion of guinea pig stomach is suitable for CRR determination; but the parameters obtained are in the range of higher concentrations than the real ones because of kinetic particularities of the preparation.
在我们之前测量分离的小鼠胃中酸分泌的实验中，我们发现组胺的EC值（引起半数最大效应的激动剂浓度）高于在分离的豚鼠右心房（收缩频率）实验中的值。这种差异可能是由于分离的小鼠胃制备中的非平衡条件或物种差异所致。为了检验这种可能性并测试在分离的分泌器官上浓度 - 反应关系（CRR）参数测定的一致性，我们比较了来自同一物种豚鼠的两种分离制剂上组胺的EC值和法莫替丁的pA值。在测量胃酸分泌时（9.7μM和6.07），EC值和pA值与在测量收缩频率的分离右心房中发现的参数（1.6μM和7.06）均有显著差异。从这些结果可以得出结论，豚鼠胃的分离部分适用于CRR测定；但由于制备的动力学特性，所获得的参数处于比实际值更高的浓度范围内。
