Lin Huiying, Li Wansha, Zhang Hao, Chen Peng, Chen Delong, He Wei, Svanberg Sune, Svanberg Katarina
Center for Optical and Electromagnetic Research, South China Normal University, Guangzhou, 510006, China.
Orthopedics Department, First Affiliated Hospital, Guangzhou University of Chinese Medicine, State Key Orthopedic Laboratory of Chinese Medicine, 16, Jichang Road, Baiyun Area, Guangzhou, 510405, China.
J Biophotonics. 2017 Oct;10(10):1356-1364. doi: 10.1002/jbio.201600229. Epub 2016 Dec 23.
Osteonecrosis of the femoral head (ONFH), a recalcitrant and disabling disease, is caused by inadequate or fully disrupted blood supply to the affected segment of the subchondral bone. Theoretically, there will develop gas-filled pores during the bone decay process due to lacking blood supply. Unfortunately, the relationship between the gas-filled pores and ONFH is still unclear. Here, we have introduced diode laser absorption spectroscopy to detect oxygen and water vapor signals in the femoral heads from hip replacement in 19 patients. Five samples are affected by osteoarthritis (OA) and the others are related to ONFH. Oxygen and water vapor signals could be obtained, demonstrating the presence of gas-filled pores in both the OA and ONFH groups while the measurement results showed no significant difference. A study of gas exchange was also performed on one excised bone sample to study how these gas pores communicate with the ambient air. The results suggested that the obtained oxygen signals inside the bone samples originate from the invasion of ambient air, which is not expected in vivo. In conclusion, the ability to detect the gas signal of laser absorption spectroscopy shows the potential for the medical application of assessing the human femoral head in vivo.
股骨头坏死（ONFH）是一种顽固且致残的疾病，由软骨下骨受影响节段的血液供应不足或完全中断引起。从理论上讲，由于缺乏血液供应，在骨坏死过程中会形成充满气体的孔隙。不幸的是，充满气体的孔隙与股骨头坏死之间的关系仍不清楚。在此，我们引入了二极管激光吸收光谱法来检测19例髋关节置换患者股骨头中的氧气和水蒸气信号。其中5个样本受骨关节炎（OA）影响，其他样本与股骨头坏死有关。可以获得氧气和水蒸气信号，这表明OA组和股骨头坏死组中均存在充满气体的孔隙，而测量结果显示无显著差异。还对一个切除的骨样本进行了气体交换研究，以研究这些气孔如何与周围空气相通。结果表明，在骨样本内部获得的氧气信号源自周围空气的侵入，而这在体内是不应出现的。总之，激光吸收光谱法检测气体信号的能力显示了其在体内评估人体股骨头方面的医学应用潜力。
