Constantino L F da S, Nascimento L B Dos S, Casanova L M, Moreira N Dos S, Menezes E A, Esteves R L, Costa S S, Tavares E S
Instituto de Biologia, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.
Instituto de Pesquisas de Produtos Naturais, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.
Photochem Photobiol Sci. 2017 Feb 15;16(2):238-245. doi: 10.1039/c6pp00343e.
Crepis japonica (L.) D.C. (Asteraceae), a weed with antioxidant, antiallergenic, antiviral and antitumor properties displays both medicinal properties and nutritional value. This study aims to assess the effects of a supplementation of blue light and UV-A radiation on the growth, leaf anatomical structure and phenolic profile of the aerial parts of Crepis japonica. Plants were grown under two light treatments: W (control - white light), W + B (white light supplemented with blue light) and W + UV-A (white light supplemented with UV-A radiation). We recorded the length, width, and weight of fresh and dry leaves, the thickness of the epidermis and mesophyll, and stomata density. The phenolic profiles of the aqueous extracts of the aerial parts were analyzed by HPLC-DAD. There was an increase in the leaf size, stomatal density, and phenolic production, and a thickening of the mesophyll and epidermis. UV-A radiation increased the phenolic production more than blue light. Blue light and UV-A radiation both improved the production of caffeic acid by about 6 and 3 times, respectively, in comparison to control. This compound was first reported as a constituent of the extract from the aerial parts together with caftaric acid. UV-A also promoted the production of chlorogenic acid (about 1.5 times in comparison to the control). We observed that the morphological and chemical parameters of C. japonica are modified in response to blue light and UV-A radiation, which can be used as tools in the cultivation of this species in order to improve its medicinal properties and nutritional value.
日本还阳参（菊科）是一种具有抗氧化、抗过敏、抗病毒和抗肿瘤特性的杂草，兼具药用价值和营养价值。本研究旨在评估补充蓝光和UV-A辐射对日本还阳参地上部分生长、叶片解剖结构和酚类物质谱的影响。将植株种植在两种光照处理下：W（对照——白光）、W + B（补充蓝光的白光）和W + UV-A（补充UV-A辐射的白光）。我们记录了鲜叶和干叶的长度、宽度和重量、表皮和叶肉的厚度以及气孔密度。通过HPLC-DAD分析地上部分水提取物的酚类物质谱。结果发现叶片大小、气孔密度和酚类物质产量增加，叶肉和表皮增厚。UV-A辐射比蓝光更能增加酚类物质的产量。与对照相比，蓝光和UV-A辐射分别使咖啡酸的产量提高了约6倍和3倍。该化合物首次作为地上部分提取物的成分与咖啡酰酒石酸一同被报道。UV-A还促进了绿原酸的产生（与对照相比约为1.5倍）。我们观察到，日本还阳参的形态和化学参数会因蓝光和UV-A辐射而发生改变，这些辐射可作为该物种栽培中的工具，以提高其药用价值和营养价值。
