Department of Pharmacy, College of Pharmacy and Natural Medicine Research Institute, Mokpo National University, Muan, Jeonnam 58554, Korea.
Department of Nursing, Dongshin University, Naju, Jeonnam 58245, Korea.
Molecules. 2018 Mar 13;23(3):650. doi: 10.3390/molecules23030650.
Leveille (Araliaceae) has been used in traditional oriental remedies for cancer, inflammation, diabetes, and thrombosis. However, a validated analytical method, standardization, and optimization of extraction conditions with respect to biological activity have not been reported. In this study, a simple and validated HPLC method for identifying and quantifying active substances in was developed. Hot water and ethanolic leaf extracts from different production regions were prepared and evaluated with regard to their chemical compositions and biological activities. The contents of active compounds such as rutin and chlorogenic acid were determined in four samples collected from different regions. The 80% ethanolic extract showed the best antioxidant activity, phenolic content, reducing power, and xanthine oxidase (XO) inhibitory activity. The validated HPLC method confirmed the presence of chlorogenic acid and rutin in leaf extracts. The antioxidant and XO inhibitory activity of extract could be attributed to the marker compounds. Collectively, these results suggest that leaves could be beneficial for the treatment or prevention of hyperuricemia-related disease, and the validated HPLC method could be a useful tool for the quality control of food or drug formulations containing .
Leveille（伞形科）在传统的东方疗法中被用于治疗癌症、炎症、糖尿病和血栓。然而，尚未有关于分析方法的验证、标准化以及针对生物活性的提取条件的优化的报告。在这项研究中，开发了一种简单且经过验证的 HPLC 方法，用于鉴定和定量中的活性物质。制备了来自不同生产地区的热水和乙醇 Leveille 叶提取物，并就其化学成分和生物活性进行了评估。从四个不同地区采集的四个样本中测定了芦丁和绿原酸等活性化合物的含量。80％乙醇提取物表现出最佳的抗氧化活性、酚含量、还原能力和黄嘌呤氧化酶（XO）抑制活性。经过验证的 HPLC 方法证实了 Leveille 叶提取物中存在绿原酸和芦丁。Leveille 提取物的抗氧化和 XO 抑制活性可归因于标记化合物。总的来说，这些结果表明 Leveille 叶可能有益于治疗或预防与高尿酸血症相关的疾病，并且经过验证的 HPLC 方法可能是含有 Leveille 的食品或药物制剂质量控制的有用工具。
