Zhao Ziqing W, White Melanie D, Bissiere Stephanie, Levi Valeria, Plachta Nicolas
Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), 61 Biopolis Drive, Singapore, 138673, Singapore.
Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Conicet, Buenos Aires, C1428EHA, Argentina.
BMC Biol. 2016 Dec 23;14(1):115. doi: 10.1186/s12915-016-0331-9.
Probing dynamic processes occurring within the cell nucleus at the quantitative level has long been a challenge in mammalian biology. Advances in bio-imaging techniques over the past decade have enabled us to directly visualize nuclear processes in situ with unprecedented spatial and temporal resolution and single-molecule sensitivity. Here, using transcription as our primary focus, we survey recent imaging studies that specifically emphasize the quantitative understanding of nuclear dynamics in both time and space. These analyses not only inform on previously hidden physical parameters and mechanistic details, but also reveal a hierarchical organizational landscape for coordinating a wide range of transcriptional processes shared by mammalian systems of varying complexity, from single cells to whole embryos.
在定量水平上探究细胞核内发生的动态过程长期以来一直是哺乳动物生物学中的一项挑战。过去十年间生物成像技术的进步使我们能够以前所未有的空间和时间分辨率以及单分子灵敏度在原位直接观察核过程。在此，我们以转录为主要关注点，综述近期的成像研究，这些研究特别强调对核动态在时间和空间上的定量理解。这些分析不仅揭示了以前隐藏的物理参数和机制细节，还揭示了一种层次化的组织格局，用于协调从单细胞到整个胚胎等不同复杂程度的哺乳动物系统所共有的广泛转录过程。
