Maibam Uttarani, Hooda O K, Sharma P S, Mohanty A K, Singh S V, Upadhyay R C
Animal Physiology Division, ICAR-National Dairy Research Institute, Karnal 132001, Haryana, India.
Animal Physiology Division, ICAR-National Dairy Research Institute, Karnal 132001, Haryana, India.
J Therm Biol. 2017 Jan;63:58-64. doi: 10.1016/j.jtherbio.2016.11.007. Epub 2016 Nov 12.
Skin is most important environmental interface providing a protective envelope to animals. It's always under the influence of both internal and external stressors. Heat shock proteins (HSP) are highly conserved stress proteins which play crucial roles in environmental stress tolerance and thermal adaptation. Present study was planned to observe the relative mRNA expression of inducible (HSP70.1 and HSP70.2) and constitutive (HSP70.8) HSP in skin of zebu (Tharparkar) and crossbred (Karan Fries) cattle during different seasons. Skin biopsies were collected from rump region of each animal, aseptically during winter, spring and summer season. Quantitative real time polymerase chain reaction was performed to examine the gene expression of constitutive (HSP70.8) and inducible (HSP70.1 and HSP70.2) HSP in skin of both the breeds during different seasons. Present study observed higher expression of both constitutive and inducible HSP genes in both the breeds during summer and winter than spring season, but magnitude of increase was higher during summer than winter. During summer season, expression pattern of HSPs in skin showed breed differences, where constitutive HSP expression was higher in Tharparkar than Karan Fries and that of inducible HSP was higher in Karan Fries than Tharparkar. Hence, present study suggested that HSP may be conveniently used as biomarkers for assessing protective response of skin against heat stress in zebu and crossbred cattle. Variation in expression between breeds is associated with their heat tolerance and thermal adaptability. In summary, skin of zebu cattle (Tharparkar) is more resistant to summer stress than crossbred (Karan Fries), providing greater protection against heat stress during summer season. Superior skin protective mechanism of zebu (Tharparkar) than crossbred (Karan-Fries) cattle against heat stress may contribute to superior adaptability of zebu cattle to tropical climatic conditions than crossbreed.
皮肤是动物最重要的环境界面，为其提供保护屏障。它始终受到内部和外部应激源的影响。热休克蛋白（HSP）是高度保守的应激蛋白，在环境应激耐受性和热适应中起关键作用。本研究旨在观察在不同季节，瘤牛（塔尔帕卡尔牛）和杂交牛（卡兰弗里斯牛）皮肤中诱导型（HSP70.1和HSP70.2）和组成型（HSP70.8）热休克蛋白的相对mRNA表达。在冬季、春季和夏季，从每头动物的臀部区域无菌采集皮肤活检样本。采用定量实时聚合酶链反应检测两个品种在不同季节皮肤中组成型（HSP70.8）和诱导型（HSP70.1和HSP70.2）热休克蛋白的基因表达。本研究观察到，两个品种在夏季和冬季的组成型和诱导型热休克蛋白基因表达均高于春季，但夏季的增加幅度高于冬季。在夏季，皮肤中热休克蛋白的表达模式存在品种差异，其中塔尔帕卡尔牛的组成型热休克蛋白表达高于卡兰弗里斯牛，而诱导型热休克蛋白在卡兰弗里斯牛中的表达高于塔尔帕卡尔牛。因此，本研究表明，热休克蛋白可方便地用作评估瘤牛和杂交牛皮肤对热应激保护反应的生物标志物。品种间表达差异与其耐热性和热适应性有关。总之，瘤牛（塔尔帕卡尔牛）的皮肤比杂交牛（卡兰弗里斯牛）更能抵抗夏季应激，在夏季提供更好的热应激保护。瘤牛（塔尔帕卡尔牛）比杂交牛（卡兰 - 弗里斯牛）具有更优越的皮肤抗热应激保护机制，这可能有助于瘤牛比杂交牛更能适应热带气候条件。
