de Andrade Pantoja Messy Hannear, Poleti Mirele Daiana, de Novais Francisco José, Duarte Kelly Kéffny Souza, Mateescu Raluca G, Mourão Gerson Barreto, Coutinho Luiz Lehmann, Fukumasu Heidge, Titto Cristiane Gonçalves
Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Av. Duque de Caxias Norte, 225, Pirassununga, 13635-900, Brazil.
Department of Animal Sciences, University of Florida, Gainesville, FL, USA.
Int J Biometeorol. 2024 Mar;68(3):435-444. doi: 10.1007/s00484-023-02602-4. Epub 2023 Dec 26.
The skin plays an important role in thermoregulation. Identification of genes on the skin that contribute to increased heat tolerance can be used to select animals with the best performance in warm environments. Our objective was to identify candidate genes associated with the heat stress response in the skin of Santa Ines sheep. A group of 80 sheep assessed for thermotolerance was kept in a climatic chamber for 8 days at a stress level temperature of 36 °C (10 am to 04 pm) and a maintenance temperature of 28 °C (04 pm to 10 am). Two divergent groups, with seven animals each, were formed after ranking them by thermotolerance using rectal temperature. From skin biopsy samples, total RNA was extracted, quantified, and used for RNA-seq analysis. 15,989 genes were expressed in sheep skin samples, of which 4 genes were differentially expressed (DE; FDR < 0.05) and 11 DE (FDR 0.05-0.177) between the two divergent groups. These genes are involved in cellular protection against stress (HSPA1A and HSPA6), ribosome assembly (28S, 18S, and 5S ribosomal RNA), and immune response (IGHG4, GNLY, CXCL1, CAPN14, and SAA-4). The candidate genes and main pathways related to heat tolerance in Santa Ines sheep require further investigation to understand their response to heat stress in different climatic conditions and under solar radiation. It is essential to verify whether these genes and pathways are present in different breeds and to understand the relationship between heat stress and other genes identified in this study.
皮肤在体温调节中发挥着重要作用。鉴定皮肤上有助于提高耐热性的基因可用于选择在温暖环境中表现最佳的动物。我们的目标是鉴定与圣伊内斯羊皮肤热应激反应相关的候选基因。将一组80只经过耐热性评估的绵羊置于气候箱中8天，应激水平温度为36°C（上午10点至下午4点），维持温度为28°C（下午4点至上午10点）。使用直肠温度按耐热性对绵羊进行排名后，形成了两个不同的组，每组七只动物。从皮肤活检样本中提取、定量总RNA，并用于RNA测序分析。在绵羊皮肤样本中表达了15,989个基因，其中两组之间有4个基因差异表达（DE；FDR < 0.05），11个基因差异表达（FDR 0.05 - 0.177）。这些基因参与细胞应激保护（HSPA1A和HSPA6）、核糖体组装（28S、18S和5S核糖体RNA）以及免疫反应（IGHG4、GNLY、CXCL1、CAPN14和SAA - 4）。与圣伊内斯羊耐热性相关的候选基因和主要途径需要进一步研究，以了解它们在不同气候条件和太阳辐射下对热应激的反应。必须验证这些基因和途径是否存在于不同品种中，并了解热应激与本研究中鉴定的其他基因之间的关系。
