Smina T P, Nitha B, Devasagayam T P A, Janardhanan K K
Amala Cancer Research Centre, Thrissur, Kerala 680 555, India; CeNTAB, SASTRA University, Thanjavur, Tamilnadu 613 401, India.
Sree Ayyappa DB College, Eramallikkra, Alappuzha 689 109, Kerala, India.
Mutat Res Genet Toxicol Environ Mutagen. 2017 Jan;813:45-51. doi: 10.1016/j.mrgentox.2016.11.010. Epub 2016 Dec 2.
Ganoderma lucidum total triterpenes were evaluated for its apoptosis-inducing and anti-cancer activities. Cytotoxicity and pro-apoptotic effect of total triterpenes were evaluated in human breast adenocarcinoma (MCF-7) cell line using MTT assay and DNA fragmentation analysis. Total triterpenes induced apoptosis in MCF-7 cells by down-regulating the levels of cyclin D1, Bcl-2, Bcl-xL and also by up-regulating the levels of Bax and caspase-9. Anti-carcinogenicity of total triterpenes was analysed using dimethyl benz [a] anthracene (DMBA) induced skin papilloma and mammary adenocarcinoma in Swiss albino mice and Wistar rats respectively. Topical application of 5mg, 10mg and 20mg total triterpenes reduced the incidence of skin papilloma by 62.5, 37.5 and 12.5% respectively. Incidence of the mammary tumour was also reduced significantly by 33.33, 66.67 and 16.67% in 10, 50 and 100mg/kg b.wt. total triterpenes treated animals respectively. Total triterpenes were also found to reduce the average number of tumours per animal and extended the tumour latency period in both the models. The results indicate the potential cytotoxicity and anti-cancerous activity of total triterpenes, there by opens up a path to the development of a safe and successive chemo preventive agent of natural origin.
对灵芝总三萜的诱导凋亡和抗癌活性进行了评估。使用MTT法和DNA片段化分析，在人乳腺腺癌（MCF-7）细胞系中评估了总三萜的细胞毒性和促凋亡作用。总三萜通过下调细胞周期蛋白D1、Bcl-2、Bcl-xL的水平以及上调Bax和caspase-9的水平来诱导MCF-7细胞凋亡。分别使用二甲基苯并[a]蒽（DMBA）诱导瑞士白化小鼠和Wistar大鼠的皮肤乳头瘤和乳腺腺癌，分析了总三萜的抗癌性。局部应用5mg、10mg和20mg总三萜分别使皮肤乳头瘤的发生率降低了62.5%、37.5%和12.5%。在分别用10、50和100mg/kg体重总三萜处理的动物中，乳腺肿瘤的发生率也分别显著降低了33.33%、66.67%和16.67%。还发现总三萜可减少每只动物的平均肿瘤数量，并延长两种模型中的肿瘤潜伏期。结果表明总三萜具有潜在的细胞毒性和抗癌活性，从而为开发一种安全且成功的天然化学预防剂开辟了道路。
