The triterpenoids from exhibit several valuable bioactivities, including antioxidant, anti-inflammatory, and anticancer properties, making them highly sought-after for applications in functional foods. To obtain more triterpenoids with higher content for subsequent application in functional food, this study firstly screened a strain with a high triterpenoid yield for the cultivation of fruiting bodies. Afterwards, the static adsorption and desorption capacities of seven macroporous adsorption resins (MARs) for fruiting body triterpenoids were evaluated via static tests, and MAR HPD-600 showed the best performance. The static adsorption kinetics and isotherms of triterpenoids were analyzed further using MAR HPD-600, revealing that the adsorption process followed pseudo-second-order kinetics and the Freundlich model, indicating a spontaneous exothermic reaction. The dynamic adsorption-desorption parameters of MAR HPD-600 were subsequently evaluated to establish the optimal enrichment process. With the optimal strategy, the content of triterpenoids in the desorption solution of MAR HPD-600 increased from 26.72 to 129.28 mg/g, with a high yield of 75.48%. Conclusively, the application of MAR presents an efficient and cost-effective technique for extracting triterpenoids, making it well-suited for functional food production requirements.