Wang C-Y, Peng Y-J, Hsu Y-J, Lee H-S, Chang Y-C, Chang C-S, Chiang S-W, Hsu Y-C, Lin M-H, Huang G-S
Department and Graduate Institute of Biology and Anatomy, National Defense Medical Center, Taipei, Taiwan; Department of Radiology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
Osteoarthritis Cartilage. 2017 Jun;25(6):976-985. doi: 10.1016/j.joca.2016.12.019. Epub 2016 Dec 21.
Chronic kidney disease (CKD) is characterized by metabolic disturbances in calcium and phosphorus homeostasis as kidney function declines. Alterations in blood perfusion in bone resulting from arteriosclerosis of bone vessels may relate to the progression of CKD. Herein, change in dynamic contrast enhanced (DCE) MRI parameters (A: amplitude, k: elimination constant, and k: permeability rate constant) and MRI T2 relaxation time of the knee cartilage were measured in a rodent nephrectomy model in order to (1) examine the relationship of peripheral blood perfusion to CKD and (2) demonstrate the feasibility of using DCE-MRI parameters and MRI T2 as imaging biomarkers to monitor disease progression.
Two groups of male Sprague-Dawley rats received either (1) no intervention or (2) 5/6 nephrectomy.
We found that the CKD group (compared with the control group) had lower A and k values and similar k value in the lateral and medial articular cartilages beginning at 12 weeks (P < 0.05); statistically significantly higher T2 values in the lateral and medial articular cartilages beginning at 18 weeks (P < 0.05); statistically significantly decreased inner luminal diameter of the popliteal artery, and altered structure of the lateral and medial articular cartilages (P < 0.05).
Perfusion deficiency and CKD may be related. DCE parameters and MRI T2 could serve as imaging biomarkers of cartilage degeneration in CKD progression.
慢性肾脏病（CKD）的特征是随着肾功能下降，钙和磷稳态出现代谢紊乱。骨血管动脉硬化导致的骨内血液灌注改变可能与CKD的进展有关。在此，我们在啮齿动物肾切除模型中测量了膝关节软骨的动态对比增强（DCE）MRI参数（A：幅度，k：消除常数，以及 kep：渗透速率常数）和MRI T2弛豫时间，以（1）研究外周血灌注与CKD的关系，以及（2）证明使用DCE-MRI参数和MRI T2作为成像生物标志物来监测疾病进展的可行性。
两组雄性Sprague-Dawley大鼠分别接受（1）无干预或（2）5/6肾切除术。
我们发现，CKD组（与对照组相比）从12周开始，外侧和内侧关节软骨的A值和k值较低，kep值相似（P < 0.05）；从18周开始，外侧和内侧关节软骨的T2值在统计学上显著更高（P < 0.05）；腘动脉的管腔内直径在统计学上显著减小，外侧和内侧关节软骨的结构改变（P < 0.05）。
灌注不足与CKD可能有关。DCE参数和MRI T2可作为CKD进展中软骨退变的成像生物标志物。
