Pasqualin Côme, Gannier François, Yu Angèle, Malécot Claire O, Bredeloux Pierre, Maupoil Véronique
Bioinformatics. 2017 Mar 1;33(5):789-790. doi: 10.1093/bioinformatics/btw754.
Simultaneous recordings of myocytes contractility and their cytoplasmic calcium concentration allow powerful studies, particularly on heart failure and other cardiac dysfunctions. Such studies require dedicated and expensive experimental devices that are difficult to use. Thus we propose SarConfoCal, the first and only software to simultaneously analyse both cytoplasmic calcium variations (from fluorescence signal) and myocytes contractility (from sarcomere length measurement) on laser scanning confocal microscopy images. SarConfoCal is easy to set up and use, especially by people without programming skills.
The software is freely distributed under the GNU General Public License. Download and setup instructions are available at http://pccv.univ-tours.fr/ImageJ/SarConfoCal . It is provided as a toolset for ImageJ (the open-source program for image analysis provided by the National Institutes of Health). SarConfoCal has been tested under Windows, Mac and Linux operating systems.
Supplementary data are available at Bioinformatics online.
同时记录心肌细胞收缩力及其细胞质钙浓度有助于开展有力的研究，尤其是针对心力衰竭和其他心脏功能障碍的研究。此类研究需要专用且昂贵的实验设备，而且使用起来难度较大。因此，我们提出了SarConfoCal软件，这是首款也是唯一一款能够在激光扫描共聚焦显微镜图像上同时分析细胞质钙变化（来自荧光信号）和心肌细胞收缩力（来自肌节长度测量）的软件。SarConfoCal易于设置和使用，尤其是对于没有编程技能的人员而言。
该软件依据GNU通用公共许可证免费分发。可在http://pccv.univ-tours.fr/ImageJ/SarConfoCal获取下载和设置说明。它作为ImageJ（美国国立卫生研究院提供的用于图像分析的开源程序）的一个工具集提供。SarConfoCal已在Windows、Mac和Linux操作系统下进行了测试。
补充数据可在《生物信息学》在线获取。
