RainbowSTORM：一个开源的 ImageJ 插件，用于光谱单分子定位显微镜（sSMLM）数据分析和图像重建。
RainbowSTORM: an open-source ImageJ plug-in for spectroscopic single-molecule localization microscopy (sSMLM) data analysis and image reconstruction.
机构信息
Department of Biomedical Engineering.
Department of Mechanical Engineering, Northwestern University, Evanston, IL 60208, USA.
出版信息
Bioinformatics. 2020 Dec 8;36(19):4972-4974. doi: 10.1093/bioinformatics/btaa635.
SUMMARY
Spectroscopic single-molecule localization microscopy (sSMLM) simultaneously captures the spatial locations and full spectra of stochastically emitting fluorescent single molecules. It provides an optical platform to develop new multimolecular and functional imaging capabilities. While several open-source software suites provide subdiffraction localization of fluorescent molecules, software suites for spectroscopic analysis of sSMLM data remain unavailable. RainbowSTORM is an open-source ImageJ/FIJI plug-in for end-to-end spectroscopic analysis and visualization for sSMLM images. RainbowSTORM allows users to calibrate, preview and quantitatively analyze emission spectra acquired using different reported sSMLM system designs and fluorescent labels.
AVAILABILITY AND IMPLEMENTATION
RainbowSTORM is a java plug-in for ImageJ (https://imagej.net)/FIJI (http://fiji.sc) freely available through: https://github.com/FOIL-NU/RainbowSTORM. RainbowSTORM has been tested with Windows and Mac operating systems and ImageJ/FIJI version 1.52.
SUPPLEMENTARY INFORMATION
Supplementary data are available at Bioinformatics online.
摘要
光谱单分子定位显微镜（sSMLM）可同时捕获随机发射荧光单分子的空间位置和全光谱。它提供了一个光学平台来开发新的多分子和功能成像能力。虽然有几个开源软件套件可提供荧光分子的亚衍射定位，但用于 sSMLM 数据光谱分析的软件套件仍然不可用。RainbowSTORM 是一个用于 sSMLM 图像的端到端光谱分析和可视化的开源 ImageJ/FIJI 插件。RainbowSTORM 允许用户使用不同报道的 sSMLM 系统设计和荧光标记校准、预览和定量分析获得的发射光谱。
可用性和实现
RainbowSTORM 是一个用于 ImageJ（https://imagej.net）/FIJI（http://fiji.sc）的 Java 插件，可通过以下网址免费获得：https://github.com/FOIL-NU/RainbowSTORM。RainbowSTORM 已经在 Windows 和 Mac 操作系统以及 ImageJ/FIJI 版本 1.52 上进行了测试。
补充信息
补充数据可在生物信息学在线获得。
Zotero 插件