Reiber H
Neurochemisches Labor, Universität Göttingen, FRG.
J Biolumin Chemilumin. 1989 Jul;4(1):245-8. doi: 10.1002/bio.1170040135.
Cellular low-level luminescence was measured after various disintegrative processes in brain cell preparations. In addition to known origins of low-level luminescence, e.g. oxygen radical reactions or enzymatic and non-enzymatic redox systems, a further source of photon emission is reported which is independent of external oxygen, oxygen radicals and enzyme activities. Vital cells from rat brain homogenates or pig oligodendrocytes could be kept for hours at 37 degrees C without any photon emission. Only after disintegrative processes a cellular photon emission could be induced. The maximal intensity of about 400 impulses/s/mg protein and a total radiation of about 6 X 10(6) l/mg depended on the type of cells. The signal could be retained completely at 4 degrees C or in frozen samples. Heating (10 min, 90 degrees C) did not suppress the photon emission. Luminol and lucigenin did not amplify the signal as is usually observed in oxygen radical-producing cells. Non-specific radical scavengers as well as detergents suppressed the cellular photon emission completely. It is suggested that this cellular luminescence represents a biophysical radiation which originates from the interruption of an intermolecular radiationless energy transfer.
在对脑细胞制剂进行各种分解过程后，测量了细胞的低水平发光。除了已知的低水平发光来源，如氧自由基反应或酶促和非酶促氧化还原系统外，还报道了一种与外部氧气、氧自由基和酶活性无关的光子发射源。大鼠脑匀浆或猪少突胶质细胞的活细胞在37℃下可保持数小时而无任何光子发射。只有在分解过程后才能诱导细胞光子发射。最大强度约为400脉冲/秒/毫克蛋白质，总辐射约为6×10⁶ 升/毫克，这取决于细胞类型。该信号在4℃或冷冻样品中可完全保留。加热（10分钟，90℃）并未抑制光子发射。鲁米诺和光泽精不像在产生氧自由基的细胞中通常观察到的那样放大信号。非特异性自由基清除剂以及去污剂完全抑制了细胞光子发射。有人认为这种细胞发光代表一种生物物理辐射，它源自分子间无辐射能量转移的中断。
